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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Using milk leukocyte differentials for diagnosis of subclinical bovine mastitis

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Autor(es):
Goncalves, Juliano Leonel ; Lyman, Roberta L. ; Hockett, Mitchell ; Rodriguez, Rudy ; dos Santos, Marcos Veiga ; Anderson, Kevin L.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF DAIRY RESEARCH; v. 84, n. 3, p. 309-317, AUG 2017.
Citações Web of Science: 4
Resumo

This research study aimed to evaluate the use of the milk leukocyte differential (MLD) to: (a) identify quarter milks that are culture-positive; and (b) characterize the milk leukocyte responses to specific groups of pathogens causing subclinical mastitis. The MLD measures the absolute number and relative percentage of inflammatory cells in milk samples. Using the MLD in two dairy herds (170 and 172 lactating cows, respectively), we studied all lactating cows with a most recent monthly Dairy Herd Improvement Association somatic cell count (SCC) >200 x 10(3) cells/ml. Quarter milk samples from 78 cows meeting study criteria were analysed by MLD and aseptically collected milk samples were subjected to microbiological culture (MC). Based upon automated instrument evaluation of the number and percentage of inflammatory cells in milk, samples were designated as either MLD-positive or - negative for subclinicial mastitis. Positive MC were obtained from 102/156 (65.4%) of MLD-positive milk samples, and 28/135 (20.7%) of MLD-negative milk samples were MC-positive. When MC was considered the gold standard for mastitis diagnosis, the calculated diagnostic Se of the MLD was 65.4% (IC95% = 57.4 to 72.8%) and the Sp was 79.3% (IC95% = 71.4 to 85.7%). Quarter milks positive on MC had higher absolute numbers of neutrophils, lymphocytes and macrophages, with higher neutrophils% and lymphocytes% but lower macrophages%. The Log(10) (N/L) ratios were the most useful ratio to differentiate specific subclinical mastitis quarters from healthy quarters. Use of the MLD on cows with monthly composite SCC > 200 x 10(3) cells/ml for screening at quarter level identified quarters more likely to be culture-positive. In conclusion, the MLD can provide an analysis of mammary quarter status more detailed than provided by SCC alone; however, the MLD response to subclinical mastitis was not found useful to specifically identify the causative pathogen. (AU)

Processo FAPESP: 13/23613-8 - Impacto da mastite subclínica sobre custo de produção e qualidade do leite em rebanhos leiteiros
Beneficiário:Juliano Leonel Gonçalves
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
Beneficiário:Bernadette Dora Gombossy de Melo Franco
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 14/17411-6 - Impacto econômico e perfil de agentes etiológicos da mastite bovina
Beneficiário:Marcos Veiga dos Santos
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/04570-1 - Impacto da mastite subclínica sobre custo de produção e qualidade do leite em rebanhos leiteiros
Beneficiário:Juliano Leonel Gonçalves
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado