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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Light source is critical to induce glioblastoma cell death by photodynamic therapy using chloro-aluminiumphtalocyanine albumin-based nanoparticles

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Autor(es):
Davanzo, Nathalia Nossi [1] ; Pellosi, Diogo Silva [1] ; Franchi, Leonardo Pereira [1] ; Tedesco, Antonio Claudio [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ctr Nanotechnol & Tissue Engn, Fac Philosophy Sci & Letters Ribeirao Preto, Photobiol & Photomed Res Grp, Dept Chem, Av Bandeirantes 3900, 14040-901 Vila Monte Alegre, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Photodiagnosis and Photodynamic Therapy; v. 19, p. 181-183, SEP 2017.
Citações Web of Science: 6
Resumo

Selection of an efficient light source is fundamental in the development of photodynamic therapy (PDT) protocols. However, few studies provide a comparison of different light sources with regard to phototoxic effects. Here, we compared the cell death induced by photoactivation of chloro-aluminiumphtalocyanine (AlClPc)-loaded human serum albumin nanoparticles under irradiation with different light sources: continuous laser (CL), pulsed laser (PL), and light -emitting diode (LED). Cells were exposed to three different AIC1Pc concentrations (1, 3, and 5 mu M) and three different light doses (200, 500, and 700 mJ/cm(2)) for each light source. Cell death and differentiation of apoptosis and necrosis pathway were measured by flow cytometry. CL was the best light source for improving the photodynamic action of AlClPc-loaded albumin nanoparticles in glioblastoma cells and avoiding undesirable side effects, especially at low photosensitizer doses (200 mJ/cm(2)). In addition, apoptosis was the main cell death pathway in all evaluated cases (70% for CL, and greater than 50% for PL and LED). In conclusion, the search for optimal light sources and light/photosensitizer doses is a crucial step in improving PDT outcomes and enhancing the clinical translation of PDT. (AU)

Processo FAPESP: 16/00389-3 - Nanopartículas multifuncionais para a entrega sítio-específica da temozolomida e verteporfina para a terapia combinada de tumores cerebrais
Beneficiário:Diogo Silva Pellosi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 14/11870-9 - Sensibilização de linhagens de câncer à terapia fotodinâmica por meio da inibição da proteína APE1
Beneficiário:Leonardo Pereira Franchi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático