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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of enamel/dentin thickness on the toxic and esthetic effects of experimental in-office bleaching protocols

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Autor(es):
de Oliveira Duque, C. C. [1] ; Soares, D. G. [2] ; Basso, F. G. [2] ; Hebling, J. [3] ; de Souza Costa, C. A. [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Dept Dent Mat & Prosthodont, Araraquara Sch Dent, Humaita St 1680, BR-14801903 Araraquara, SP - Brazil
[2] Univ Estadual Paulista UNESP, Dept Physiol & Pathol, Araraquara Sch Dent, Humaita St 1680, BR-14801903 Araraquara, SP - Brazil
[3] Univ Estadual Paulista UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, Humaita St 1680, BR-14801903 Araraquara, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: CLINICAL ORAL INVESTIGATIONS; v. 21, n. 8, p. 2509-2520, NOV 2017.
Citações Web of Science: 10
Resumo

This paper aims to assess the whitening effectiveness and toxicity of tooth-bleaching protocols applied to enamel/dentin disks simulating mandibular incisors (ICs) and premolars (PMs). A 10% hydrogen peroxide (H2O2) gel was applied for 3 x 15, 1 x 15, or 1 x 5 min to enamel/dentin disks simulating mandibular ICs and PMs, and the trans-enamel and trans-dentinal diffusion products were applied to human dental pulp cells (1 h). Professional therapy (35% H2O2-3 x 15 min) was used as positive control, and non-bleached samples were used as negative control. Cell viability and morphology, oxidative stress generation, and odontoblastic marker expression were assessed. The H2O2 diffusion and enamel color change (Delta E) were also analyzed. The 10% H2O2 gel induced significant cell viability reduction only when applied 3 x 15 min, with the intensity of oxidative stress and down-regulation of odontoblastic markers being higher in the IC group. The other experimental bleaching protocols caused slight alterations regarding the cell parameters evaluated, with intensity being related to enamel/dentin thickness. These effects were also correlated with higher H2O2 diffusion in the IC group. Delta E values similar as positive control were found for the 10% 3 x 15 and 1 x 15 protocols on IC group, after 4 and 6 sessions. Application of a 10% H2O2 bleaching gel for 15 or 45 min to thin dental substrate significantly minimizes cell toxicity in comparison with highly concentrated gels associated with similar esthetic outcomes by increasing the number of bleaching sessions. Bleaching gels with 10% H2O2 applied in small teeth for short periods may be an interesting alternative to obtain whitening effectiveness without causing toxicity to pulp cells, which may be able to reduce the tooth hypersensitivity claimed by patients. (AU)

Processo FAPESP: 14/07229-6 - Efeito da espessura do esmalte e dentina sobre a eficácia estética e citotoxicidade de diferentes técnicas de clareamento dental
Beneficiário:Carla Caroline de Oliveira Duque
Modalidade de apoio: Bolsas no Brasil - Mestrado