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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column

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Autor(es):
da Silva, Meire Ribeiro [1, 2] ; Brandtzaeg, Ole Kristian [2] ; Vehus, Tore [2, 3] ; Lancas, Fernando Mauro [1] ; Wilson, Steven Ray [2] ; Lundanes, Elsa [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Chem Sao Carlos, Sao Carlos, SP - Brazil
[2] Univ Oslo, Dept Chem, Post Box 1033, NO-0315 Oslo - Norway
[3] Univ Agder, Dept Engn Sci, Grimstad - Norway
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Chromatography A; v. 1518, p. 104-110, OCT 6 2017.
Citações Web of Science: 5
Resumo

An open tubular (OT) sample preparation/separation platform was developed. A multi-channel polymer layer open tubular (mPLOT) solid phase extraction (SPE) column was prepared by wall-coating the 126 channels (8 mu m inner diameter (ID) each) of a crystal fiber capillary with an organic polymer, namely poly(styrene-co-octadecene-co-divinylbenzene) (PS-OD-DVB). The mPLOT SPE was coupled online with a 10 mu m x 2m poly(styrene-co-divinylbenzene) (PS-DVB) OT liquid chromatography column with nanospray mass spectrometry (OTLC-MS). Compared to using monolithic/particle-packed SPEs, mPLOT-SPE-OTLC allowed both fast loading and sufficient refocusing on the OT analytical column of small model compounds (sulfonamides approximate to 300 Da). Using automatic filtration/filter back-flushing (AFFL) plumbing, the mPLOT SPE column gave a constant and low back-pressure approximate to 35 bar at 0.5 mu L/min. Surprisingly large sample volumes (10 mu L) were possible to be injected using a 12 cm mPLOT. (C) 2017 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 15/12306-2 - Preparação e avaliação de colunas tubulares abertas porosas (plot) para separação de sulfonamidas e seus metabólitos acetilados em amostras alimentícias por nano-LC-MS/MS
Beneficiário:Meire Ribeiro da Silva
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado