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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Luxurious Nitrogen Fertilization of Two Sugar Cane Genotypes Contrasting for Lignin Composition Causes Changes in the Stem Proteome Related to Carbon, Nitrogen, and Oxidant Metabolism but Does Not Alter Lignin Content

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Autor(es):
Salvato, Fernanda [1, 2] ; Wilson, Rashaun [3] ; Portilla Llerena, Juan Pablo [1] ; Kiyota, Eduardo [1] ; Reis, Karina Lima [2] ; Boaretto, Luis Felipe [2] ; Balbuena, Tiago S. [4] ; Azevedo, Ricardo A. [2] ; Thelen, Jay J. [3] ; Mazzafera, Paulo [2, 1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083862 Campinas, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil
[3] Univ Missouri, Dept Biochem, Columbia, MO 65201 - USA
[4] Univ Estadual Paulista Julio de Mesquita, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PROTEOME RESEARCH; v. 16, n. 10, p. 3688-3703, OCT 2017.
Citações Web of Science: 1
Resumo

Sugar cane is an important crop for sugar and biofuel production. Its lignocellulosic biomass represents a promising option as feedstock for second-generation ethanol production. Nitrogen fertilization can affect differently tissues and its biopolymers, including the cell-wall polysaccharides and lignin. Lignin content and composition are the most important factors associated with biomass recalcitrance to convert cell-wall polysaccharides into fermentable sugars. Thus it is important to understand the metabolic relationship between nitrogen fertilization and lignin in this feedstock. In this study, a large-scale proteomics approach based on GeLC-MS/MS was employed to identify and relatively quantify proteins differently accumulated in two contrasting genotypes for lignin composition after excessive nitrogen fertilization. From the similar to 1000 nonredundant proteins identified, 28 and 177 were differentially accumulated in response to nitrogen from IACSP04-065 and IACSP04-627 lines, respectively. These proteins were associated with several functional categories, including carbon metabolism, amino acid metabolism, protein turnover, and oxidative stress. Although nitrogen fertilization has not changed lignin content, phenolic acids and lignin composition were changed in both species but not in the same way. Sucrose and reducing sugars increased in plants of the genotype IACSP04-065 receiving nitrogen. (AU)

Processo FAPESP: 14/25994-1 - Lignina em espécies de Saccharum spp.
Beneficiário:Juan Pablo Portilla Llerena
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/10779-5 - Proteoma do colmo e do núcleo de células de cana-de-açúcar (Saccharum spp.) associado ao controle da biossíntese de lignina
Beneficiário:Fernanda Salvato
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/06236-4 - Identificação e quantificação absoluta de fatores de transcrição envolvidos na biossíntese de lignina em colmos de cana-de-açúcar submetidos a estresse hídrico
Beneficiário:Fernanda Salvato
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático