Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Carbohydrate-active enzymes in Trichoderma harzianum: a bioinformatic analysis bioprospecting for key enzymes for the biofuels industry

Texto completo
Autor(es):
Ferreira Filho, Jaire Alves [1, 2] ; Crivelente Horta, Maria Augusta [1] ; Beloti, Lilian Luzia [1] ; dos Santos, Clelton Aparecido [1] ; de Souza, Anete Pereira [1, 3]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Ctr Mol Biol & Genet Engn CBMEG, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Grad Program Genet & Mol Biol, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BMC Genomics; v. 18, OCT 12 2017.
Citações Web of Science: 2
Resumo

Background: Trichoderma harzianum is used in biotechnology applications due to its ability to produce powerful enzymes for the conversion of lignocellulosic substrates into soluble sugars. Active enzymes involved in carbohydrate metabolism are defined as carbohydrate-active enzymes (CAZymes), and the most abundant family in the CAZy database is the glycoside hydrolases. The enzymes of this family play a fundamental role in the decomposition of plant biomass. Results: In this study, the CAZymes of T. harzianum were identified and classified using bioinformatic approaches after which the expression profiles of all annotated CAZymes were assessed via RNA-Seq, and a phylogenetic analysis was performed. A total of 430 CAZymes (3.7% of the total proteins for this organism) were annotated in T. harzianum, including 259 glycoside hydrolases (GHs), 101 glycosyl transferases (GTs), 6 polysaccharide lyases (PLs), 22 carbohydrate esterases (CEs), 42 auxiliary activities (AAs) and 46 carbohydrate-binding modules (CBMs). Among the identified T. harzianum CAZymes, 47% were predicted to harbor a signal peptide sequence and were therefore classified as secreted proteins. The GH families were the CAZyme class with the greatest number of expressed genes, including GH18 (23 genes), GH3 (17 genes), GH16 (16 genes), GH2 (13 genes) and GH5 (12 genes). A phylogenetic analysis of the proteins in the AA9/GH61, CE5 and GH55 families showed high functional variation among the proteins. Conclusions: Identifying the main proteins used by T. harzianum for biomass degradation can ensure new advances in the biofuel production field. Herein, we annotated and characterized the expression levels of all of the CAZymes from T. harzianum, which may contribute to future studies focusing on the functional and structural characterization of the identified proteins. (AU)

Processo FAPESP: 16/19775-0 - Desenvolvimento / suplementação de coquetéis enzimáticos destinados à hidrólise de biomassa vegetal: utilização de mutagênese sítio-dirigida e proteínas quiméricas
Beneficiário:Clelton Aparecido dos Santos
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 15/09202-0 - Estudo de regiões genômicas de Trichoderma harzianum associadas ao controle da expressão de enzimas envolvidas na degradação de biomassa
Beneficiário:Anete Pereira de Souza
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/18856-1 - Estudo da expressão gênica global e de regiões genômicas associadas ao controle da expressão de enzimas envolvidas na degradação de compostos lignocelulósicos por Trichoderma harzianum
Beneficiário:Maria Augusta Crivelente Horta
Linha de fomento: Bolsas no Brasil - Pós-Doutorado