Photobiomodulation therapy action in wound repair skin induced in aged rats old: time course of biomarkers inflammatory and repair

Autor(es)

Fiorio, Franciane Barbieri ^[1] ; dos Santos, Solange Almeida ^[1] ; de Melo Rambo, Caroline Sobral ^[1] ; Dalbosco, Camila Guerra ^[2] ; Serra, Andrey Jorge ^[3] ; de Melo, Brunno Lemes ^[4] ; Pinto Leal-Junior, Ernesto Cesar ^[1] ; Camillo de Carvalho, Paulo de Tarso ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Nove de Julho UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[2] Chapeco Univ UNOCHAPECO, Hlth Sci Ctr, Chapeco, SC - Brazil

^[3] Nove de Julho Univ UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Rua Vergueiro 235, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Postgrad Program Med Cardiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 32, n. 8, p. 1769-1782, NOV 2017.
• Citações Web of Science: 5

Resumo

Previous studies have discussed an inverse correlation between age and wound healing, because it relates to the association of aging with a gradual decrease in healing capacity. Treatment with photobiomodulation therapy (PBMT) improves wound healing by inducing increases in mitotic activity, numbers of fibroblasts, collagen synthesis, and neovascularization. Therefore, this study aimed to evaluate the effects of PBMT in cutaneous wound healing in aged rats. A punch biopsy of 8 mm in diameter was performed to produce a skin wound. The study included 45 male rats, of which 15 were young (30 days) and 30 were elderly (500 days). The 45 animals were distributed into 3 experimental groups, which were subjected to skin wounds and 1 aged group received PBMT, with a 30-mW laser beam (power density of 1.07 W/cm(2)), beam area of 0.028 cm(2), and lambda 660 nm produced through active phosphide Gallium-Aluminum-Indio (InGaAIP). The PBMT application took the form of a single-point transcutaneous method, with a total energy of 2 joules per wound site, energy density of 72 J/cm(2), and time of 1 min and 7 s. Analysis was performed to verify the effect of PBMT on the quantity of collagen I and III, metalloproteinase 3 and 9 (MMP-3 and MMP-9), tissue inhibitor of metalloproteinase-2 (TIMP-2) and of vascular endothelial growth factor (VEGF) at the wound site by immunohistochemistry, cytokine-induced neutrophil chemoattractant (CINC)-1, by enzyme-linked immunosorbent assay (ELISA) and interleukin (IL)-6 real-time polymerase chain reaction (RT-PCR). That we conclude LLLT is effective in the modulation of inflammatory mediators IL-6, CINC-1, VEGF, MMP-3, MMP-9 and TIMP-2 as well as increased collagen production in aged animals during different phases of the tissue regeneration process. However, the effects of PBMT obtained in the aged animals (aged LLLT group) suggest that new dosimetries should be tested to achieve better results. (AU)

• Processo FAPESP: 15/13677-4 - Efeitos da Laserterapia de Baixa Intensidade na Biodisponibilidade de Células-Tronco Mesenquimais Derivadas de Adipócitos e Implantadas em Joelhos de Ratos Submetidos a Osteoartrite Experimental
• Beneficiário: Paulo de Tarso Camillo de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular