Pro-inflammatory Analysis of Macrophages in Contact with Titanium Particles and Porphyromonas gingivalis

Autor(es)

Cindy Goes Dodo ^[1] ; Luiz Meirelles ^[2] ; Alejandro Aviles-Reyes ^[3] ; Karina Gonzalez Silvério Ruiz ^[4] ; Jacqueline Abranches ^[5] ; Altair Antoninha Del Bel Cury ^[6]

Afiliação do(s) autor(es)

^[1] Universidade Estadual de Campinas - Brasil

^[2] American Dental Association - Estados Unidos

^[3] University of Florida - Estados Unidos

^[4] Universidade Estadual de Campinas - Brasil

^[5] University of Florida - Estados Unidos

^[6] Universidade Estadual de Campinas - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 28, n. 4, p. 428-434, 2017-08-00.

Resumo

Abstract During insertion of titanium dental implants, particles may shear from the implant to the periimplant region causing osteolysis, and their association with bacteria can exacerbate the inflammatory reaction. However, the association of a high invasive bacterium from the oral cavity, Porphyromonas gingivalis (Pg), and titanium particles remains unknown. This study evaluated pro-inflammatory reaction of human macrophages in contact with micro and nanoparticles of titanium associated with Porphyromonas gingivalis lipopolysaccharide (PgLPS). THP-1 cell were used and treated for 12, 24 and 48 h following 6 groups: Control(C), PgLPS (L); Microparticles (M); Nanoparticles (N); PgLPS and microparticles (LM); PgLPS and nanoparticles (LN). The following assays were carried out: i) cell viability using MTS, ii) cell morphology by SEM and iii) expression of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by qRT-PCR and ELISA. For statistics two-way ANOVA followed by Tukey’s test was used (p<0.05). After treatment, cells presented similar viability and morphology demonstrating that the treatments were not able to induce cell death. Gene expression was significantly higher for TNF-α and IL1-β after 12 h, and for IL-6 after 24 h in the N and LN groups. Cytokine production over time was an ascending curve for TNF-α with the peak at 48 h and IL1-β and IL-6 had a straight line among the time points, although cells from N group presented a significant production of IL-6 at 48 h. In conclusion, these results suggest that titanium nanoparticles stimulate stronger pro-inflammatory response in macrophages, independent of their association with LPS from P.gingivalis. (AU)

• Processo FAPESP: 13/19791-8 - Análise proteômica da membrana celular de fibroblastos gengivais humanos cultivados sobre diferentes superfícies de titânio e avaliação do uso de antioxidante exógeno para controle do estresse oxidativo celular
• Beneficiário: Cindy Goes Dodo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/10085-6 - Análise celular de macrófagos em contato com nanopartículas de óxido de titânio
• Beneficiário: Cindy Goes Dodo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto