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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Adhesion and invasion of Clostridium perfringens type A into epithelial cells

Texto completo
Autor(es):
Llanco, Luis A. [1] ; Nakano, Viviane [1] ; de Moraes, Claudia T. P. [2] ; Piazza, Roxane M. F. [2] ; Avila-Campos, Mario J. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Anaerobe Lab, Sao Paulo, SP - Brazil
[2] Butantan Inst, Bacteriol Lab, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Microbiology; v. 48, n. 4, p. 764-768, OCT-DEC 2017.
Citações Web of Science: 3
Resumo

Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and alpha- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL () induced strong cell rounding after 6 h of incubation and produced cell enlargement. None of the 22 strains harbored netB gene. All the six tpeL (+) gene strains were able to adhere to HEp-2 cells; however, only four of them (66.6%) were invasive. Thus, these results suggest that the presence of tpeL gene or TpeL toxin might be required for the adherence of bacteria to HEp-2 cells; however, it could not have any role in the invasion process. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. (AU)

Processo FAPESP: 13/17739-9 - Avaliação clonal de cepas de Clostridium perfringens isolados de humanos e animais por Multilocus sequence typing (MLST)
Beneficiário:Mario Júlio Ávila-Campos
Linha de fomento: Auxílio à Pesquisa - Regular