Susceptibility of mice to bovine herpesvirus type 5 infection in the central nervous system

Autor(es)

Mesquita, L. P. ^[1] ; Costa, R. C. ^[1] ; Fusuma, M. M. ^[2] ; Bruhn, F. R. P. ^[3] ; Mori, E. ^[4] ; Pituco, E. M. ^[2] ; Mori, C. M. C. ^[1] ; Weiblen, R. ^[5] ; Maiorka, P. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] Biol Inst, Lab Bovine Viral Dis, Sao Paulo - Brazil

^[3] Univ Fed Pelotas, Coll Vet Med, Pelotas - Brazil

^[4] Pasteur Inst, Sao Paulo - Brazil

^[5] Univ Fed Santa Maria, Dept Vet Prevent Med, Santa Maria, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY RESEARCH COMMUNICATIONS; v. 41, n. 4, p. 279-288, DEC 2017.
• Citações Web of Science: 0

Resumo

Bovine herpesvirus type 5 (BoHV-5) is an important pathogen that causes meningoencephalitis in cattle. Few studies have used the mouse as a model for BoHV-5 infection. Despite the fact that BoHV-5 can infect mice with immune deficiencies, little is known about viral replication, immune response, and the course of infection in the central nervous system (CNS) of wild-type mice. Therefore, the aim of this study was to evaluate the response in the CNS of BALB/c mice acutely infected with BoHV-5 at different days post-inoculation (dpi). BoHV-5, when inoculated intracranially, was able to infect and replicate within the CNS of BALB/c mice. Until 15 dpi, the mice were able to survive without showing prominent neurological signs. The infection was accompanied by a Th1 immune response, with a significant expression of the cytokines IFN-gamma and TNF-alpha and chemokine CCL-2. The expression of these cytokines and chemokines was most significant in the early course of infection (3 and 4 dpi), and it was followed by meningoencephalitis with perivascular cuffing and periventriculitis, composed mainly of macrophages and lymphocytes. After the expression of cytokines and chemokine, the mice were able to curb BoHV-5 acute infection in the brain, since there was a decrease in the number of BoHV-5 DNA copies after 3 dpi and viable viral particles were not detected after 6 dpi. Importantly, BoHV-5 was able to infect the trigeminal ganglia during acute infection, since a large number of BoHV-5 DNA copies were detected on 1 and 2 dpi. (AU)

• Processo FAPESP: 12/24769-9 - Identificação de marcadores da resposta imune e inflamatória em encefalites virais induzidas experimentalmente em um modelo murino
• Beneficiário: Paulo César Maiorka
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/00629-6 - Avaliação da Resposta Imune e Inflamatória na Encefalite Experimentalmente Induzida em Camundongos pela Infecção por Herpesvírus Bovino Tipo 5 (BHV-5) e Vírus da Estomatite Vesicular (VEV).
• Beneficiário: Leonardo Pereira Mesquita
• Modalidade de apoio: Bolsas no Brasil - Doutorado