Production of three symbiosis-related fatty acids by Symbiodinium types in clades A-F associated with marine invertebrate larvae

Autor(es)

Mies, M. ^[1] ; Chaves-Filho, A. B. ^[2] ; Miyamoto, S. ^[2] ; Guth, A. Z. ^[1] ; Tenorio, A. A. ^[1] ; Castro, C. B. ^{[3, 4]} ; Pires, D. O. ^{[3, 4]} ; Calderon, E. N. ^{[4, 5]} ; Sumida, P. Y. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Oceanog Inst, Praca Oceanog 191, BR-05508120 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Chem Inst, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Fed Rio de Janeiro, Museu Nacl, Quinta Boa Vista S-N, BR-20940040 Rio De Janeiro, RJ - Brazil

^[4] Inst Coral Vivo, Rua Coqueiros 87, BR-45807000 Santa Cruz Cabralia, BA - Brazil

^[5] Univ Fed Rio de Janeiro, Nucl Ecol & Desenvolvimento Socioambiental Macae, Av Sao Jose Barreto 764, BR-27965045 Macae, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CORAL REEFS; v. 36, n. 4, p. 1319-1328, DEC 2017.
• Citações Web of Science: 5

Resumo

Symbiodinium are dinoflagellates engaged in a mutualistic symbiosis with multiple coral reef taxa. They are divided in nine different clades (A-I), which typically associate with different hosts. However, very little information is available on metabolic differences in Symbiodinium types, especially when associated with metazoan larvae. We tested whether three omega 3 fatty acids (stearidonic acid, SDA; docosapentaenoic acid, DPA; and docosahexaenoic acid, DHA) that are typically translocated from Symbiodinium to its host are produced by Symbiodinium types within clades A-F associated with Mussismilia hispida (scleractinian coral), Berghia stephanieae (nudibranch), and Tridacna crocea (giant clam) larvae. We acquired and spawned broodstock for each host, cultured their larvae, and offered Symbiodinium types belonging to clades A-F. Samples were taken during a 72-h window after the offer of Symbiodinium, and fatty acids were extracted and analyzed by gas chromatography. The concentrations of SDA and DPA for all host larvae-dinoflagellate associations were low and variable, without trends. However, M. hispida planula larvae associated with Symbiodinium A1 and C1 had a statistically significant higher amount of DHA. The veliger larvae of B. stephanieae digested the Symbiodinium, and the amount of DHA remained constant throughout the experiment. The veliger larvae of T. crocea associated with Symbiodinium A1 and C1 also presented a higher amount of DHA, although not statistically different from the other types. These results show that Symbiodinium A1 and C1, in the case of M. hispida and T. crocea (which usually harbor strains within clades A and C), may contribute a small amount of DHA to the larvae of these organisms and form a stronger mutualism than other strains. (AU)

• Processo FAPESP: 10/20350-8 - Efeitos da acidificação no metabolismo e trofodinâmica do bentos marinho
• Beneficiário: Paulo Yukio Gomes Sumida
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Regular