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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Negative Slope Conductance of the Persistent Sodium Current Prolongs Subthreshold Depolarizations

Texto completo
Autor(es):
Ceballos, Cesar C. [1, 2] ; Roque, Antonio C. [2] ; Leao, Ricardo M. [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Sch Philosophy Sci & Letters, Dept Phys, Ribeirao Preto - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BIOPHYSICAL JOURNAL; v. 113, n. 10, p. 2207-2217, NOV 21 2017.
Citações Web of Science: 5
Resumo

Neuronal subthreshold voltage-dependent currents determine membrane properties such as the input resistance (Rin) and the membrane time constant (tm) in the subthreshold range. In contrast with classical cable theory predictions, the persistent sodium current (INaP), a non-inactivating mode of the voltage-dependent sodium current, paradoxically increases Rin and tm when activated. Furthermore, this current amplifies and prolongs synaptic currents in the subthreshold range. Here, using a computational neuronal model, we showed that the creation of a region of negative slope conductance by INaP activation is responsible for these effects and the ability of the negative slope conductance to amplify and prolong Rin and tm relies on the fast activation of INaP. Using dynamic clamp in hippocampal CA1 pyramidal neurons in brain slices, we showed that the effects of INaP on Rin and tm can be recovered by applying an artificial INaP after blocking endogenous INaP with tetrodotoxin. Furthermore, we showed that injection of a pure negative conductance is enough to reproduce the effects of INaP on Rin and tm and is also able to prolong artificial excitatory post synaptic currents. Since both the negative slope conductance and the almost instantaneous activation are critical for producing these effects, the INaP is an ideal current for boosting the amplitude and duration of excitatory post synaptic currents near the action potential threshold. (AU)

Processo FAPESP: 13/07699-0 - Centro de Pesquisa, Inovação e Difusão em Neuromatemática - NeuroMat
Beneficiário:Jefferson Antonio Galves
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 16/01607-4 - Estimulação auditiva de alta intensidade e plasticidade sináptica
Beneficiário:Ricardo Mauricio Xavier Leão
Linha de fomento: Auxílio à Pesquisa - Regular