Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In vitro schistosomicidal and antiviral activities of Arctium lappa L. (Asteraceae) against Schistosoma mansoni and Herpes simplex virus-1

Texto completo
Autor(es):
Mostrar menos -
Dias, Mirna Meana [1] ; Zuza, Ohana [1] ; Riani, Lorena R. [1] ; Pinto, Priscila de Faria [2] ; Silva Pinto, Pedro Luiz [3] ; Silva, Marcos P. [4] ; de Moraes, Josue [4] ; Ataide, Ana Caroline Z. [5] ; Silva, Fernanda de Oliveira [5] ; Cecilio, Alzira Batista [5] ; Da Silva Filho, Ademar A. [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Fed Juiz de Fora, Dept Pharmaceut Sci, R Jose Lourenco Kelmer S-N, Campus Univ, BR-36036900 Juiz De Fora, MG - Brazil
[2] Univ Fed Juiz de Fora, Inst Biol Sci, Dept Biochem, Juiz De Fora, MG - Brazil
[3] Adolfo Lutz Inst, Nucleo Enteroparasitas, Sao Paulo - Brazil
[4] Univ Guarulhos, Nucleo Pesquisa Doencas Negligenciadas, Guarulhos, SP - Brazil
[5] Fundacao Ezequiel Dias, Diretoria Pesquisa & Desenvolvimento, Serv Biotecnol & Saude, Belo Horizonte, MG - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 94, p. 489-498, OCT 2017.
Citações Web of Science: 10
Resumo

Schistosomiasis and herpes diseases represent serious issues to the healthcare systems, infecting a large number of people worldwide, mainly in developing countries. Arctium lappa L. (Asteraceae), known as ``bardana{''} and ``burdock{''}, is a medicinal plant popularly used for several purposes, including as antiseptic. In this study, we evaluated the in vitro schistosomicidal and antiherpes activities of the crude extract of A. lappa, which have not yet been described. Fruits of A. lappa L. were extracted by maceration with ethanol: H2O (96: 4 v/v) in order to obtain the hydroalcoholic extract of A. lappa (AL). In vitro schistosomicidal assays were assessed against adult worms of Schistosoma mansoni, while the in vitro antiviral activity of AL was evaluated on replication of Herpes simplex virus type-1 (HSV-1). Cell viability was measured by MTT assay, using Vero cells and chemical composition of AL was determined by qualitative UPLC-ESI-QTOF-MS analysis. UPLC-ESI-QTOF-MS analysis of AL revealed the presence of dibenzylbutyrolactone lignans, such as arctiin and arctigenin. Results showed that AL was not cytotoxic to Vero cells even when tested at 400 mu g/mL. qPCR results indicated a significant viral load decreased for all tested concentrations of AL (400, 50, and 3.125 mu g/mL), which showed similar antiviral effect to acyclovir (50 mu g/mL) when tested at 400 mu g/mL. Also, AL (400, 200, and 100 mu g/mL) caused 100% mortality and significantly reduction on motor activity of all adult worms of S. mansoni. Confocal laser scanning microscopy showed tegumental morphological alterations and changes on the numbers of tubercles of S. mansoni worms in a dose-dependent manner after treatment with AL. This report provides the first evidence for the in vitro schistosomicidal and antiherpes activities of AL, opening the route to further schistosomicidal and antiviral studies with AL and their compounds, especially lignans. (C) 2017 Elsevier Masson SAS. All rights reserved. (AU)

Processo FAPESP: 16/22488-3 - Reposicionamento de fármacos para doenças negligenciadas: identificação de novos agentes anti-helmínticos
Beneficiário:Josué de Moraes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 00/11624-5 - Embriologia de Gastropoda: análise em microscopia de varredura laser confocal
Beneficiário:Toshie Kawano
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários