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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Methionine-supplemented diet affects the expression of cardiovascular disease-related genes and increases inflammatory cytokines in mice heart and liver

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Autor(es):
Aissa, Alexandre Ferro [1] ; do Amaral, Catia Lira [2] ; Venancio, Vinicius Paula [3] ; Machado, Carla da Silva [1] ; Hernandes, Livia Cristina [3] ; da Silva Santos, Patrick Wellington [3] ; Curi, Rui [4] ; Pires Bianchi, Maria de Lourdes [3] ; Greggi Antunes, Lusania Maria [3]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Estadual Goias, Campus Henrique Santillo, Anapolis, GO - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 80, n. 19-21, SI, p. 1116-1128, 2017.
Citações Web of Science: 2
Resumo

Some important environmental factors that influence the development of cardiovascular diseases (CVD) include tobacco, excess alcohol, and unhealthy diet. Methionine obtained from the diet participates in the synthesis of DNA, proteins, lipids and affects homocysteine levels, which is associated with the elevated risk for CVD development. Therefore, the aim of this study was to investigate the manner in which dietary methionine might affect cellular mechanisms underlying CVD occurrence. Swiss albino mice were fed either control (0.3% DL-methionine), methionine-supplemented (2% DL-methionine), or a methionine-deprived diet (0% DL-methionine) over a 10-week period. The parameters measured included plasma homocysteine concentrations, oxidative stress by reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, levels of inflammatory cytokines IL-1 beta, TNF-alpha, and IL-6, as well as expression of genes associated with CVD. The levels of apolipoprotein A5 (APOA5), a regulator of plasma triglycerides, were measured. The methionine-supplemented diet increased oxidative stress by lowering the GSH/GSSG ratio in heart tissues and decreased expression of the genes Apob, Ctgf, Serpinb2, Spp1, Il1b, andSell, but elevated expression of Thbs4, Tgfb2, Ccr1, andVegfa. Methionine-deprived diet reduced expression of Col3a1, Cdh5, Fabp3, Bax, andHbegf and increased expression of Sell, Ccl5, Itga2, Birc3, Msr1, Bcl2a1a, Il1r2, and Selp. Methionine-deprived diet exerted pro-inflammatory consequences as evidenced by elevated levels of cytokines IL-1 beta, TNF-alpha, and IL-6 noted in liver. Methionine-supplemented diet increased hepatic IL-6 and cardiac TNF-alpha. Both methionine supplementation and deprivation lowered hepatic levels of APOA5. In conclusion, data demonstrated that a methionine-supplemented diet modulated important biological processes associated with high risk of CVD development. (AU)

Processo FAPESP: 13/02365-6 - Avaliação do perfil de metilação, instabilidade genômica e expressão de RNAm de genes relacionados com doenças cardiovasculares em camundongos suplementados com metionina
Beneficiário:Lusânia Maria Greggi Antunes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/01410-0 - Interação gene-dieta em camundongos suplementados com metionina nos períodos fetal e pós-natal: estudos de instabilidade genômica, metilação e expressão de genes relacionados com doenças cardiovasculares
Beneficiário:Alexandre Ferro Aissa
Linha de fomento: Bolsas no Brasil - Doutorado