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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Paper-Based Microfluidics Immunoassay for Detection of Canine Distemper Virus

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Autor(es):
Mazzu-Nascimento, Thiago [1, 2] ; Donofrio, Fabiana Cristina [3] ; Bianchi, Beatriz Cutilak [4] ; Travensolo, Regiane de Fatima [4] ; Souza Junior, Joao Batista [1] ; Moraes, Daniel Angeli [1] ; Morbioli, Giorgio Gianini [1, 2, 5] ; Segato, Thiago Pinotti [4] ; Varanda, Laudemir Carlos [1] ; Carrilho, Emanuel [1, 2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
[2] Inst Nacl Ciencia & Tecnol Bioanalit, Campinas, SP - Brazil
[3] Univ Fed Mato Grosso, Inst Ciencias Saude, BR-78557267 Sinop, MT - Brazil
[4] Partecurae Pesquisa & Desenvolvimento, BR-13560460 Sao Carlos, SP - Brazil
[5] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Brazilian Archives of Biology and Technology; v. 60, p. -, 2017.
Citações Web of Science: 1
Resumo

ABSTRACT Paper-based devices present low-cost and are versatile, making them very attractive for clinical analysis. To manufacture those devices wax patterns are printed on paper surface and upon heating the wax permeates through the entire thickness of the paper, creating hydrophobic barriers that delimit test areas. Antibodies produced in rabbits against canine distemper virus (CDV) were physically adsorbed on the surface of gold nanoparticles (AuNPs) and incubated with CDV viral antigens, forming the immunocomplex. Anti-CDV antibodies were immobilized into the microchannels by physical adsorption, forming the test region. The test solution containing conjugated AuNPs was applied at the bottom of the microchannel and it was eluted with a phosphate buffer solution 0.01 M pH 7.4. When the solution containing the AuNPs reached the test zone the recognition of antigens contained on the immunocomplex occurred with the consequent development of a red line, which represents a positive outcome for the test. This method demonstrated the success of physical immobilization of antibodies on AuNPs and the physical immobilization of antibodies on cellulose’s surface. This colorimetric assay brings simplicity and versatility to clinical analyses, presenting potential for CDV diagnosis. (AU)

Processo FAPESP: 12/02093-3 - Síntese de nanoestruturas core@shell de Co@Au (superparamagnéticas) e CdSe@ZnS (quantum dots) visando aplicações antineoplásicas
Beneficiário:João Batista Souza Junior
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/13997-8 - Plataformas em papel para detecção não invasiva de biomarcadores tumorais e para cultivo de células neoplásicas
Beneficiário:Thiago Mazzu Do Nascimento
Linha de fomento: Bolsas no Brasil - Doutorado Direto