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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Placental and colostral transfer of antibodies reactive with enteropathogenic Escherichia coli intimins alpha, beta, or gamma

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Autor(es):
Altman, Silvia P. N. [1, 2] ; Tino-De-Franco, Milene [1] ; Carbonare, Cristiane B. [1] ; Palmeira, Patricia [3] ; Carbonare, Solange B. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Imunogenet, Sao Paulo, SP - Brazil
[2] Hosp Israelita Albert Einstein, Sao Paulo, SP - Brazil
[3] Hosp Clin Sao Paulo, Inst Crianca, Lab Invest Med LIM 36, Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Jornal de Pediatria; v. 93, n. 6, p. 568-575, NOV-DEC 2017.
Citações Web of Science: 3
Resumo

Objective: Intimins are protein adhesins of enteropathogenic Escherichia coli and enterohemorrhagic E. coli capable of inducing attachment and effacement lesions in enterocytes. Anti-intimin antibodies are important for the protection from enteropathogenic E. coli and enterohemorrhagic E. coli infections because these antibodies inhibit bacterial adhesion and impair the initial step of the pathogenesis. We studied the transfer of maternal anti-intimin antibodies from healthy Brazilian mothers to their newborns through the placenta and colostrum. Methods: Serum immunoglobulin G and secretory immunoglobulin A antibodies against conserved and variable regions of intimins alpha, beta, and gamma were analyzed using an enzyme linked-immunosorbent assay in the blood and colostrum from 45 healthy women as well as cord blood serum samples from their newborns. Results: The concentrations of antibodies reactive with alpha intimin were significantly lower than those of anti-gamma and anti-conserved intimin antibodies in the colostrum samples. IgG serum antibodies reactive with all the subtypes of intimins were transferred to the newborns, but the concentrations of anti-conserved intimin serum antibodies were significantly higher in mothers and newborns than concentrations of antibodies against variable regions. The patterns of IgG transfer from mothers to newborns were similar for all anti-intimin antibodies. These values are similar to the percentage transference of total IgG. Conclusions: Anti-intimin antibodies are transferred from mothers to newborns through the placenta, and reinforce the protection provided by breastfeeding against diarrheagenic E. coli infections. (c) 2017 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. (AU)

Processo FAPESP: 03/13250-3 - Avaliação da atividade in vitro e in vivo de anticorpos humanos séricos e secretores anti-Escherichia coli enteropatogênicas e Escherichia coli produtoras de toxinas Shiga
Beneficiário:Solange Barros Carbonare
Linha de fomento: Auxílio à Pesquisa - Regular