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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Biochemical characterization and low-resolution SAXS structure of an exopolygalacturonase from Bacillus licheniformis

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Autor(es):
Evangelista, Danilo Elton [1] ; de Araujo, Evandro A. [1] ; Oliveira Neto, Mario [2] ; Seiki Kadowaki, Marco Antonio [1] ; Polikarpov, Igor [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil
[2] Univ Estadual Paulista Julio De Mesquita Filho UN, Inst Biociencias, Dept Fis & Biofis, BR-18618970 Botucatu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: NEW BIOTECHNOLOGY; v. 40, n. B, p. 268-274, JAN 25 2017.
Citações Web of Science: 3
Resumo

Among the structural polymers present in the plant cell wall, pectin is the main component of the middle lamella. This heterogeneous polysaccharide has an alpha-1,4 galacturonic acid backbone, which can be broken by the enzymatic action of pectinases, such as exo-polygalacturonases, that sequentially cleave pectin from the nonreducing ends, releasing mono or di-galacturonic acid residues. Constant demand for pectinases that better suit industrial requirements has motivated identification and characterization of novel enzymes from diverse sources. Bacillus licheniformis has been used as an important source for bioprospection of several industrial biomolecules, such as surfactants and enzymes, including pectate lyases. Here we cloned, expressed, purified, and biochemically and structurally characterized an exo-polygalacturonase from B. licheniformis (BlExoPG). Its low-resolution molecular envelope was derived from experimental small-angle scattering data (SAXS). Our experimental data revealed that BlExoPG is a monomeric enzyme with optimum pH at 6.5 and optimal temperature of approximately 60 degrees C, at which it has considerable stability over the broad pH range from 5 to 10. After incubation of the enzyme for 30 min at pH ranging from 5 to 10, no significant loss of the original enzyme activity was observed. Furthermore, the enzyme maintained residual activity of greater than 80% at 50 degrees C after 15 h of incubation. BlExoPG is more active against polygalacturonic acid as compared to methylated pectin, liberating mono galacturonic acid as a unique product. Its enzymatic parameters are V-max = 4.18 mu M. s(-1), K-m = 3.25 mgmL(-1) and k(cat)= 2.58 s(-1). (AU)

Processo FAPESP: 15/13684-0 - Estudos estruturais e funcionais de enzimas que participam na síntese e degradação de carboidratos complexos
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/20505-4 - Duas classes importantes de glicosil hidrolases: estudos funcionais e análise estrutural
Beneficiário:Marco Antonio Seiki Kadowaki
Linha de fomento: Bolsas no Brasil - Pós-Doutorado