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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pseudomonas spp.: contamination sources in bulk tanks of dairy farms

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Autor(es):
Vidal, Ana M. C. [1] ; Netto, Arlindo Saran [2] ; Vaz, Andreia C. N. [1] ; Capodifoglio, Eduardo [2] ; Goncalves, Ana C. S. [3] ; Rossi, Gabriel A. M. [3] ; Figueiredo, Annanda S. [1] ; Ruiz, Vera L. A. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FZEA, Dept Med Vet, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, FZEA, Dept Zootecnia, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[3] Univ Estadual Paulista Unesp, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Rodovia Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Pesquisa Veterinária Brasileira; v. 37, n. 9, p. 941-948, SEP 2017.
Citações Web of Science: 1
Resumo

This study focused on isolating Pseudomonas spp. during milking process in ten dairy farms with manual and mechanical milking systems during dry and rainy seasons, and evaluating DNA homology and patterns of distribution between isolates, in order to identify main sources of milk contamination by Pseudomonas spp. A total of 167 isolates of Pseudomonas spp. were obtained from water, milkers' hands, cows' teats, teat cups, cooling tanks and raw milk. Bacteria of Pseudomonas spp. genus were isolated from 85 and 82 sampling points in dairy farms with manual and mechanical milking system, respectively. A significant difference (p= 0.02) on Pseudomonas spp. isolation was observed among samples of surface of cows' teats before and after pre-dipping, but no significant difference (p> 0.05) was observed among milking systems or seasons. The possibility of the same Pseudomonas spp. patterns are distributed in different farms and seasons using Amplified Fragment Length Polymorphism (AFLP) technique was demonstrated. Milkers' hands, surface of cows' teats, teat cups and cooling tanks were associated with raw milk contamination with Pseudomonas spp. on farms with manual and mechanical milking system, showing that regardless of the type of milking system and season, proper hygiene procedures of equipment, utensils and workers' hands are essential to avoid contamination of the milk and, therefore, improve milk quality. (AU)

Processo FAPESP: 13/04131-2 - Avaliação da qualidade microbiológica, contagem de Pseudomonas spp. e sua importância durante a obtenção e armazenamento de leite cru refrigerado tipo b no período de seca e chuva
Beneficiário:Ana Maria Centola Vidal
Linha de fomento: Auxílio à Pesquisa - Regular