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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation

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Autor(es):
Cruz-Silva, Ilana [1] ; Nunes, Viviane Abreu [2] ; Gozzo, Andrezza Justino [3] ; Praxedes-Garcia, Priscila [1] ; Tanaka, Aparecida Sadae [1] ; Shimamoto, Kazuaki [4] ; Araujo, Mariana Silva [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Biochem, Rua Tres de Maio 100, BR-04044020 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Arts Sci & Humanities, Ave Arlindo Bettio 1000, BR-03828000 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Marine Sci, Rua Doutor Carvalho de Mendonca 144, BR-11070100 Santos, SP - Brazil
[4] Japan Hlth Care Coll, Kiyota Ku, Sinei 434-1, Sapporo, Hokkaido - Japan
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PULMONARY MEDICINE; 2016.
Citações Web of Science: 1
Resumo

Inflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an in vivo lung inflammation model in rats, in the presence or absence of CeKI (C. echinata kallikrein inhibitor), a plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, and rCeEI (recombinant C. echinata elastase inhibitor), which inhibits these proteases and also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative control) or inhibitors and, subsequently, lipopolysaccharide was injected into their lungs. Blood, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. In plasma, kinin release was higher in the LPS-treated animals in comparison to CeKI or rCeEI groups. rCeEI-treated animals presented less kinin than CeKI-treated group. Our data suggest that kinins play a pivotal role in lung inflammation and may be generated by different enzymes; however, neutrophil elastase seems to be the most important in the lung tissue context. These results open perspectives for a better understanding of biological process where neutrophil enzymes participate and indicate these plant inhibitors and their recombinant correlates for therapeutic trials involving pulmonary diseases. (AU)

Processo FAPESP: 04/11015-0 - Modulação do sistema calicreína-cininas e de seus receptores, influência de glicosaminoglicanos, inibidores e doenças neurodegenerativas
Beneficiário:Mariana da Silva Araujo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 01/02457-0 - Papel da catepsina "b" em celulas tumorais - efeito de inibidores na sua atividade e no seu transito.
Beneficiário:Andrezza Justino Gozzo Andreotti
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 07/55496-0 - Proteases, inibidores naturais e recombinantes de sementes de Caesalpinia echinata (pau-brasil): interações em processos inflamatórios e neurodegenerativos, com modulação por glicosaminoglicanos
Beneficiário:Mariana da Silva Araujo
Linha de fomento: Auxílio à Pesquisa - Regular