Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photodynamic damage predominates on different targets depending on cell growth phase of Candida albicans

Texto completo
Autor(es):
Baptista, Alessandra [1, 2] ; Sabino, Caetano P. [3, 2] ; Nunez, Silvia C. [1] ; Miyakawa, Walter [4] ; Martin, Airton A. [1] ; Ribeiro, Martha S. [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Brasil, Biomed Engn Postgrad Program, Sao Paulo, SP - Brazil
[2] CNEN SP, IPEN, Nucl & Energy Res Inst, Ctr Lasers & Applicat, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
[4] Inst Adv Studies, Photon Div, Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 177, p. 76-84, DEC 2017.
Citações Web of Science: 5
Resumo

Photodynamic inactivation (PDI) has been reported to be effective to eradicate a wide variety of pathogens, including antimicrobial-resistant microorganisms. The aim of this study was to identify the potential molecular targets of PDI depending on growth phase of Candida albicans. Fungal cells in lag (6 h) and stationary (48 h) phases were submitted to PDI mediated by methylene blue (MB) combined with a (662 +/- 21) nm-LED, at 360 mW of optical power. Pre-irradiation time was 10 min and exposure times were 12 min, 15 min and 18 min delivering radiant exposures of 129.6 J/cm(2), 162 J/cm(2) and 194.4 J/cm(2), respectively, on a 24-well plate of about 2 cm(2) at an irradiance of 180 mW/cm(2). Scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force spectroscopy (AFS) and Fourier transform infrared spectroscopy (FT-IR) were employed to evaluate the photodynamic effect in young and old fungal cells following 15 min of irradiation. Morphological analysis revealed wrinkled and shrunk fungal cell membrane for both growth phases while extracellular polymeric substance (EPS) removal was only observed for old fungal cells. Damaged intracellular structures were more pronounced in young fungal cells. The surface nanostiffness of young fungal cells decreased after PDI but increased for old fungal cells. Cellular adhesion force was reduced for both growth phases. Fungal cells in lag phase predominantly showed degradation of nucleic acids and proteins, while fungal cells in stationary phase showed more pronounced degradation of polysaccharides and lipids. Taken together, our results indicate different molecular targets for fungal cells in lag and stationary growth phase following PDI. (AU)

Processo FAPESP: 10/13313-9 - Desenvolvimento de metodologias para o estudo do efeito fotodinâmico em infecções fúngicas
Beneficiário:Martha Simões Ribeiro
Linha de fomento: Auxílio à Pesquisa - Regular