Transcriptomic profile reveals molecular events associated to focal adhesion and invasion in canine mammary gland tumour cell lines

Autor(es)

Cordeiro, Y. G. ^[1] ; Xavier, P. L. P. ^[1] ; Rochetti, A. L. ^[1] ; Alexandre, P. A. ^[1] ; Mori, C. M. C. ^[2] ; Strefezzi, R. F. ^[1] ; Fukumasu, H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, Lab Comparat & Translat Oncol, Ave Duque de Caxias Norte 255, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY AND COMPARATIVE ONCOLOGY; v. 16, n. 1, p. E89-E98, MAR 2018.
• Citações Web of Science: 1

Resumo

The prevalence of cancer in animals has increased significantly over the years. Mammary tumours are the most common neoplasia in dogs, in which around 50% are presented in the malignant form. Hence, the development and characterization of in vitro models for the study of canine tumours are important for the improvement of cancer diagnosis and treatment. Thus, the aim of this study was to characterize cell lines derived from canine mammary gland neoplasias which could be further used for basic and applied oncology research. Samples of canine mammary carcinomas were taken for cell culture and 2 cell lines were established and characterized in terms of cell morphology, tumourigenicity and global gene expression. Both cell lines presented spindle-shape morphology and shown common malignant features as in vitro invasion potential and expression of epithelial and mesenchymal proteins. Also, we found gene expression patterns between the 2 cell cultures in comparison to the normal mammary gland tissue. Cells from M25 culture showed a higher invasion and in vivo tumourigenic potential, associated to the overexpression of genes involved in focal adhesion and extracellular matrix communication, such as FN1, ITGA8 and THBS2. The phenotypic characterization of these cells along with their global gene expression profile potentially determine new therapeutic targets for mammary tumours. (AU)

• Processo FAPESP: 14/02493-7 - Neoplasias mamárias de cadelas e a teoria das células-tronco cancerosas: uma abordagem comparada e translacional
• Beneficiário: Heidge Fukumasu
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores