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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes

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Autor(es):
Zubieta, Mariane Paludetti [1] ; Contesini, Fabiano Jares [1] ; Rubio, Marcelo Ventura [1] ; de Souza Schmidt Goncalves, Any Elisa [2] ; Gerhardt, Jaqueline Aline [1] ; Prade, Rolf Alexander [3] ; de Lima Damasio, Andre Ricardo [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, Campinas, SP - Brazil
[3] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: MICROBIAL BIOTECHNOLOGY; v. 11, n. 2, p. 346-358, MAR 2018.
Citações Web of Science: 4
Resumo

Filamentous fungi are robust cell factories and have been used for the production of large quantities of industrially relevant enzymes. However, the production levels of heterologous proteins still need to be improved. Therefore, this article aimed to investigate the global proteome profiling of Aspergillus nidulans recombinant strains in order to understand the bottlenecks of heterologous enzymes production. About 250, 441 and 424 intracellular proteins were identified in the control strain Anid\_pEXPYR and in the recombinant strains Anid\_AbfA and Anid\_Cbhl respectively. In this context, the most enriched processes in recombinant strains were energy pathway, amino acid metabolism, ribosome biogenesis, translation, endoplasmic reticulum and oxidative stress, and repression under secretion stress (RESS). The global protein profile of the recombinant strains Anid\_AbfA and Anid\_Cbhl was similar, although the latter strain secreted more recombinant enzyme than the former. These findings provide insights into the bottlenecks involved in the secretion of recombinant proteins in A.nidulans, as well as in regard to the rational manipulation of target genes for engineering fungal strains as microbial cell factories. (AU)

Processo FAPESP: 12/20549-4 - Secreção de glicoproteínas heterólogas em Aspergillus: efeito do padrão de glicosilação em parâmetros funcionais de glicosil hidrolases
Beneficiário:André Ricardo de Lima Damasio
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores