Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Divergent brain changes in two audiogenic rat strains: A voxel-based morphometry and diffusion tensor imaging comparison of the genetically epilepsy prone rat (GEPR-3) and the Wistar Audiogenic Rat (WAR)

Texto completo
Autor(es):
Lee, Yichien [1, 2, 3] ; Rodriguez, Olga C. [1, 2, 3] ; Albanese, Chris [1, 2, 3, 4] ; Santos, Victor Rodrigues [5] ; Cortes de Oliveira, Jose Antonio [6] ; Ferreira Donatti, Ana Luiza [6] ; Fernandes, Artur [6, 7] ; Garcia-Cairasco, Norberto [6] ; N'Gouemo, Prosper [8, 9] ; Forcelli, Patrick A. [5, 8, 10]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC - USA
[2] Georgetown Univ, Preclin Res Imaging Lab, Washington, DC - USA
[3] Georgetown Univ, Dept Pathol, Washington, DC - USA
[4] Georgetown Univ, Dept Oncol, Washington, DC - USA
[5] Georgetown Univ, Dept Pharmacol Physiol, Washington, DC - USA
[6] Univ Sao Paulo, Rtheirdo Preto Sch Med, Dept, Neurophysiol & Expt Neuroethol Lab LNNE, Ribeirao Preto - Brazil
[7] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Genet, Lab Epigenet & Reprod, Ribeirao Preto - Brazil
[8] Georgetown Univ, Interdisciplinary Program Neurosci, Washington, DC - USA
[9] Georgetown Univ, Dept Pediat, Washington, DC - USA
[10] Georgetown Univ, Dept Neurosci, Washington, DC - USA
Número total de Afiliações: 10
Tipo de documento: Artigo Científico
Fonte: Neurobiology of Disease; v. 111, p. 80-90, MAR 2018.
Citações Web of Science: 5
Resumo

Acoustically evoked seizures (e.g., audiogenic seizures or AGS) are common in models of inherited epilepsy and occur in a variety of species including rat, mouse, and hamster. Two models that have been particularly well studied are the genetically epilepsy prone rat (GEPR-3) and the Wistar Audiogenic Rat (WAR) strains. Acute and repeated AGS, as well as comorbid conditions, displays a close phenotypic overlap in these models. Whether these similarities arise from convergent or divergent structural changes in the brain remains unknown. Here, we examined the brain structure of Sprague Dawley (SD) and Wistar (WIS) rats, and quantified changes in the GEPR-3 and WAR, respectively. Brains from adult, male rats of each strain (n = 8-10 per group) were collected, fixed, and embedded in agar and imaged using a 7 tesla Bruker MRI. Post-acquisition analysis included voxel-based morphometry (VBM), diffusion tensor imaging (DTI), and manual volumetric tracing. In the VBM analysis, GEPR-3 displayed volumetric changes in brainstem structures known to be engaged by AGS (e.g., superior and inferior colliculus, periaqueductal grey) and in forebrain structures (e.g., striatum, septum, nucleus accumbens). WAR displayed volumetric changes in superior colliculus, and a broader set of limbic regions (e.g., hippocampus, amygdala/piriform cortex). The only area of significant overlap in the two strains was the midline cerebellum: both GEPR-3 and WAR showed decreased volume compared to their control strains. In the DTI analysis, GEPR-3 displayed decreased fractional anisotropy (FA) in the corpus callosum, posterior commissure and commissure of the inferior colliculus (IC). WAR displayed increased FA only in the commissure of IC. These data provide a biological basis for further comparative and mechanistic studies in the GEPR-3 and WAR models, as well as provide additional insight into commonalities in the pathways underlying AGS susceptibility and behavioral comorbidity. (AU)

Processo FAPESP: 07/50261-4 - Neurociências, epilepsia e arte: caracterização interdisciplinar da cepa WAR e modelos de epilepsia correlacionados: as oficinas Da Vinci, divulgação científica e quebras de estigmas em epilepsia
Beneficiário:Norberto Garcia Cairasco
Linha de fomento: Auxílio à Pesquisa - Temático