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In silico characterization of tandem repeats in Trichophyton rubrum and related dermatophytes provides new insights into their role in pathogenesis

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Autor(es):
Franco, Matheus Eloy [1, 2] ; Bitencourt, Tamires Aparecida [1, 3] ; Marins, Mozart [1, 4] ; Fachin, Ana Lucia [1, 4]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Ribeirao Preto, Unidade Biotecnol, Av Costabile Romano 2201, BR-14096900 Ribeirao Preto, SP - Brazil
[2] Fed Inst Educ Sci & Technol South Minas Gerais IF, BR-37750000 Belo Horizonte, MG - Brazil
[3] Univ Sao Paulo, FMRP, Dept Genet, BR-049900 Sao Paulo, SP - Brazil
[4] Univ Ribeirao Preto, Curso Med, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION; JUN 11 2017.
Citações Web of Science: 0
Resumo

Trichophyton rubrum is the most common etiological agent of dermatophytoses worldwide, which is able to degrade keratinized tissues. The sequencing of the genome of different dermatophyte species has provided a large amount of data, including tandem repeats that may play a role in genetic variability and in the pathogenesis of these fungi. Tandem repeats are adjacent DNA sequences of 2-200 nucleotides in length, which exert regulatory and adaptive functions. These repetitive DNA sequences are found in different classes of fungal proteins, especially those involved in cell adhesion, a determinant factor for the establishment of fungal infection. The objective of this study was to develop a Dermatophyte Tandem Repeat Database (DTRDB) for the storage and identification of tandem repeats in T. rubrum and six other dermatophyte species. The current version of the database contains 35 577 tandem repeats detected in 16 173 coding sequences. The repeats can be searched using entry parameters such as repeat unit length (nt-nucleotide), repeat number, variability score, and repeat sequence motif. These data were used to study the relative frequency and distribution of repeats in the sequences, as well as their possible functions in dermatophytes. A search of the database revealed that these repeats occur in 22-33% of genes transcribed in dermatophytes where they could be involved in the success of adaptation to the host tissue and establishment of infection. The repeats were detected in transcripts that are mainly related to three biological processes: regulation, adhesion, and metabolism. The database developed enables users to identify and analyse tandem repeat regions in target genes related to pathogenicity and fungal-host interactions in dermatophytes and may contribute to the discovery of new targets for the development of antifungal agents. (AU)

Processo FAPESP: 12/03845-9 - Transcriptoma do dermatófito Trichophyton rubrum em resposta ao antifúngico transchalcona em condições de cultivo que simulam a infecção da pele humana
Beneficiário:Ana Lucia Fachin Saltoratto
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/23841-3 - Estudo de compostos naturais com atividade antifúngica com vistas ao elucidamento dos mecanismos de ação
Beneficiário:Ana Lucia Fachin Saltoratto
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/02920-7 - Transcriptoma do dermatófito Trichophyton rubrum em resposta ao antifúngico transchalcona em condições de cultivo que simulam a infecção
Beneficiário:Tamires Aparecida Bitencourt
Linha de fomento: Bolsas no Brasil - Doutorado