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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization

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Autor(es):
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Moraes, Eduardo C. [1] ; Alvarez, Thabata M. [2] ; Persinoti, Gabriela F. [1] ; Tomazetto, Geizecler [1] ; Brenelli, Livia B. [1] ; Paixao, Douglas A. A. [1] ; Ematsu, Gabriela C. [1] ; Aricetti, Juliana A. [1] ; Caldana, Camila [1] ; Dixon, Neil [3] ; Bugg, Timothy D. H. [4] ; Squina, Fabio M. [5]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, Campinas - Brazil
[2] UP, Master Program Ind Biotechnol, Curitiba, Parana - Brazil
[3] Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester, Lancs - England
[4] Univ Warwick, Dept Chem, Coventry, W Midlands - England
[5] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Sorocaba - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: BIOTECHNOLOGY FOR BIOFUELS; v. 11, MAR 22 2018.
Citações Web of Science: 10
Resumo

Background: Lignin is a heterogeneous polymer representing a renewable source of aromatic and phenolic bioderived products for the chemical industry. However, the inherent structural complexity and recalcitrance of lignin makes its conversion into valuable chemicals a challenge. Natural microbial communities produce biocatalysts derived from a large number of microorganisms, including those considered unculturable, which operate synergistically to perform a variety of bioconversion processes. Thus, metagenomic approaches are a powerful tool to reveal novel optimized metabolic pathways for lignin conversion and valorization. Results: The lignin-degrading consortium (LigMet) was obtained from a sugarcane plantation soil sample. The LigMet taxonomical analyses (based on 16S rRNA) indicated prevalence of Proteobacteria, Actinobacteria and Firmicutes members, including the Alcaligenaceae and Micrococcaceae families, which were enriched in the LigMet compared to sugarcane soil. Analysis of global DNA sequencing revealed around 240,000 gene models, and 65 draft bacterial genomes were predicted. Along with depicting several peroxidases, dye-decolorizing peroxidases, laccases, carbohydrate esterases, and lignocellulosic auxiliary (redox) activities, the major pathways related to aromatic degradation were identified, including benzoate (or methylbenzoate) degradation to catechol (or methylcatechol), catechol orthocleavage, catechol meta-cleavage, and phthalate degradation. A novel Paenarthrobacter strain harboring eight gene clusters related to aromatic degradation was isolated from LigMet and was able to grow on lignin as major carbon source. Furthermore, a recombinant pathway for vanillin production was designed based on novel gene sequences coding for a feruloyl-CoA synthetase and an enoyl-CoA hydratase/aldolase retrieved from the metagenomic data set. Conclusion: The enrichment protocol described in the present study was successful for a microbial consortium establishment towards the lignin and aromatic metabolism, providing pathways and enzyme sets for synthetic biology engineering approaches. This work represents a pioneering study on lignin conversion and valorization strategies based on metagenomics, revealing several novel lignin conversion enzymes, aromatic-degrading bacterial genomes, and a novel bacterial strain of potential biotechnological interest. The validation of a biosynthetic route for vanillin synthesis confirmed the applicability of the targeted metagenome discovery approach for lignin valorization strategies. (AU)

Processo FAPESP: 15/23279-6 - Análise metagenômica e metasecretômica de um consórcio microbiano enriquecido de fungos anaeróbicos degradador de bagaço de cana: prospecção de celulossomas e enzimas lignocelulolíticas
Beneficiário:Geizecler Tomazetto
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/03061-0 - Caracterização de frações de lignina extraídas do bagaço de cana-de-açúcar com propriedades antioxidantes
Beneficiário:Lívia Beatriz Brenelli de Paiva
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/50590-4 - Valorização da lignina em plantas de etanol celulósico: a conversão biocatalítica via ácido felúrico a produtos químicos de alto valor
Beneficiário:Fábio Márcio Squina
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 14/26152-4 - Prospecção de enzimas e otimização de vias metabólicas para produção de vanilina a partir de lignina do bagaço de cana de açúcar
Beneficiário:Eduardo Cruz Moraes
Linha de fomento: Bolsas no Brasil - Doutorado