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The effects of aging on biosynthetic processes in the rat hypothalamic osmoregulatory neuroendocrine system

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Greenwood, Michael P. [1] ; Greenwood, Mingkwan [1] ; Romanova, Elena V. [2, 3] ; Mecawi, Andre S. [4, 5, 6] ; Paterson, Alex [1] ; Sarenac, Olivera [1, 7] ; Japundzic-Zigon, Nina [7] ; Antunes-Rodrigues, Jose [5] ; Paton, Julian F. R. [8] ; Sweedler, Jonathan V. [2, 3] ; Murphy, David [4, 1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Bristol, Sch Clin Sci, Bristol, Avon - England
[2] Univ Illinois, Dept Chem, Urbana, IL - USA
[3] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL - USA
[4] Univ Malaya, Dept Physiol, Kuala Lumpur - Malaysia
[5] Univ Sao Paulo, Sch Med Ribeirao Preto, Ribeirao Preto - Brazil
[6] Univ Fed Rural Rio de Janeiro, Inst Biol & Hlth Sci, Dept Physiol Sci, Seropedica - Brazil
[7] Univ Belgrade, Fac Med, Inst Pharmacol Clin Pharmacol & Toxicol, Belgrade - Serbia
[8] Univ Bristol, Sch Physiol & Pharmacol, Bristol, Avon - England
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: NEUROBIOLOGY OF AGING; v. 65, p. 178-191, MAY 2018.
Citações Web of Science: 0

Elderly people exhibit a diminished capacity to cope with osmotic challenges such as dehydration. We have undertaken a detailed molecular analysis of arginine vasopressin (AVP) biosynthetic processes in the supraoptic nucleus (SON) of the hypothalamus and secretory activity in the posterior pituitary of adult (3 months) and aged (18 months) rats, to provide a comprehensive analysis of age-associated changes to the AVP system. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, we identified differences in pituitary peptides, including AVP, in adult and aged rats under both basal and dehydrated states. In the SON, increased Avp gene transcription, coincided with reduced Avp promoter methylation in aged rats. Based on transcriptome data, we have previously characterized a number of novel dehydration-induced regulatory factors involved in the response of the SON to osmotic cues. We found that some of these increase in expression with age, while dehydration induced expression of these genes in the SON was attenuated in aged rats. In summary, we show that aging alters the rat AVP system at the genome, transcriptome, and peptidome levels. These alterations however did not affect circulating levels of AVP in basal or dehydrated states. (C) 2018 The Authors. Published by Elsevier Inc. (AU)

Processo FAPESP: 13/09799-1 - Regulação da homeostase energética e do balanço hidromineral: das células aos sistemas fisiológicos
Beneficiário:José Antunes Rodrigues
Linha de fomento: Auxílio à Pesquisa - Temático