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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes

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Autor(es):
Florentino, Pilar T. V. [1] ; Real, Fernando [2] ; Orikaza, Cristina M. [3] ; da Cunha, Julia P. C. [4] ; Vitorino, Francisca N. L. [4] ; Cordero, Esteban M. [1, 5] ; Sobreira, Tiago J. P. [6] ; Mortara, Renato A. [3]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil
[2] INSERM, Inst Cochin, U1016, Paris - France
[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
[4] Butantan Inst, Immune Response & Cell Signaling CeTICS, Ctr Toxins, Special Lab Cell Cycle, Sao Paulo - Brazil
[5] Univ Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago - Chile
[6] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 9, APR 10 2018.
Citações Web of Science: 2
Resumo

Trypanosoma cruzi is the etiologic agent of Chagas' disease. It is known that amastigotes derived from trypomastigotes in the extracellular milieu are infective in vitro and in vivo. Extracellular amastigotes (EAs) have a stage-specific surface antigen called Ssp-4, a GPI-anchored glycoprotein that is secreted by the parasites. By immunoprecipitation with the Ssp-4-specific monoclonal antibodies (mAb) 2C2 and 1D9, we isolated the glycoprotein from EAs. By mass spectrometry, we identified the core protein of Ssp-4 and evaluated mRNA expression and the presence of Ssp-4 carbohydrate epitopes recognized by mAb1D9. We demonstrated that the carbohydrate epitope recognized by mAb1D9 could promote host cell invasion by EAs. Although infectious EAs express lower amounts of Ssp-4 compared with less-infectious EAs (at the mRNA and protein levels), it is the glycosylation of Ssp-4 (identified by mAb1D9 staining only in infectious strains and recognized by galectin-3 on host cells) that is the determinant of EA invasion of host cells. Furthermore, Ssp-4 is secreted by EAs, either free or associated with parasite vesicles, and can participate in host-cell interactions. The results presented here describe the possible role of a carbohydrate moiety of T. cruzi surface glycoproteins in host cell invasion by EA forms, highlighting the potential of these moieties as therapeutic and vaccine targets for the treatment of Chagas' disease. (AU)

Processo FAPESP: 11/51475-3 - Biologia molecular e celular do parasitismo por Trypanosoma cruzi
Beneficiário:José Franco da Silveira Filho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/23150-5 - Caracterização estrutural e funcional da glicoproteína Ssp-4 de formas amastigotas de Trypanosoma cruzi
Beneficiário:Pilar Sampaio Tavares Veras
Linha de fomento: Bolsas no Brasil - Doutorado Direto