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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

DUOX1 Silencing in Mammary Cell Alters the Response to Genotoxic Stress

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Autor(es):
Fortunato, Rodrigo S. [1, 2] ; Gomes, Luciana R. [2] ; Munford, Veridiana [2] ; Pessoa, Carolina Fittipaldi [1] ; Quinet, Annabel [2] ; Hecht, Fabio [1, 3] ; Kajitani, Gustavo S. [2] ; Milito, Cristiane Bedran [4] ; Carvalho, Denise P. [3] ; Martins Menck, Carlos Frederico [2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Radiobiol Mol, Rio De Janeiro, RJ - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Reparo DNA, Sao Paulo, SP - Brazil
[3] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Fisiol Endocrina Doris Rosenthal, Rio De Janeiro, RJ - Brazil
[4] Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Dept Patol, Rio De Janeiro, RJ - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; 2018.
Citações Web of Science: 2
Resumo

DUOX1 is an H2O2-generating enzyme related to a wide range of biological features, such as hormone synthesis, host defense, cellular proliferation, and fertilization. DUOX1 is frequently downregulated in lung and liver cancers, suggesting a tumor suppressor role for this enzyme. Here, we show that DUOX1 expression is decreased in breast cancer cell lines and also in breast cancers when compared to the nontumor counterpart. In order to address the role of DUOX1 in breast cells, we stably knocked down the expression of DUOX1 in nontumor mammary cells (MCF12A) with shRNA. This led to higher cell proliferation rates and decreased migration and adhesion properties, which are typical features for transformed cells. After genotoxic stress induced by doxorubicin, DUOX1-silenced cells showed reduced IL-6 and IL-8 secretion and increased apoptosis levels. Furthermore, the cell proliferation rate was higher in DUOX1-silenced cells after doxorubicin medication in comparison to control cells. In conclusion, we demonstrate here that DUOX1 is silenced in breast cancer, which seems to be involved in breast carcinogenesis. (AU)

Processo FAPESP: 13/21075-9 - Participação da NADPH oxidase 2 induzida pelo estrogênio em lesões no DNA e na regulação da autofagia em linhagem celular de mama
Beneficiário:Carlos Frederico Martins Menck
Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Brasil
Processo FAPESP: 14/15982-6 - Consequências de deficiências de reparo de lesões no genoma
Beneficiário:Carlos Frederico Martins Menck
Linha de fomento: Auxílio à Pesquisa - Temático