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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Free Amino Acids Analysis in the Venom of the Social Wasp Polistes lanio Under Different Forms of Preservation

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Autor(es):
Torres, Viviana de Oliveira [1, 2] ; Piva, Raul Cremonezi [2] ; Antonialli Junior, William Fernando [1, 2] ; Lima Cardoso, Claudia Andrea [1, 2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Mato Grosso Sul UEMS, Lab Ecol Comportamental, Programa Posgrad Recursos Nat, Rodovia Dourados Itahum, Km 12, Caixa Postal 351, BR-79804970 Dourados, MS - Brazil
[2] Univ Estadual Mato Grosso Sul UEMS, Rodovia Dourados Itahum, Km 12, Caixa Postal 351, Ctr Estudos Recursos Nat, BR-79804970 Dourados, MS - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ORBITAL-THE ELECTRONIC JOURNAL OF CHEMISTRY; v. 10, n. 1, SI, p. 1-8, JAN 2018.
Citações Web of Science: 2
Resumo

Venoms of social wasps have in their composition proteins, peptides, among others and several of these compounds were evaluated for their function in the venom, however, there is still no study of the quantification of free amino acids of venom of social wasps. The peptides and proteins of the venom can originate in the medium amino acids or other molecules by the rupture of the peptide bonds. Therefore, the objective was evaluated the composition of the free amino acid in venom of the social wasp Polistes lanio and validated a method for free amino acids analysis using different forms of preservation of this venom. For this, the venom of P. lanio was analyzed direct and partitioned (liquid-liquid with organic solvents) by High Performance Liquid Chromatography. For validation of the amino acids, parameters as linearity, stability and recovery of the assay method were evaluated by the analyzing it fresh or frozen, and before or after extraction with organic solvents. The free amino acids were recovered from these samples at rates ranging from 93.2-99.0%. The data obtained indicate that the freezing, storage and extraction with organic solvents does not lead to an increase in the amino acids content of proteins and peptides. (AU)

Processo FAPESP: 08/57701-2 - Bases tecnológicas para identificação, síntese e uso de semioquímicos na agricultura
Beneficiário:José Roberto Postali Parra
Linha de fomento: Auxílio à Pesquisa - Temático