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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of Regulatory Immune Response in Skin Lesions of Patients Affected by Nonulcerated or Atypical Cutaneous Leishmaniasis in Honduras, Central America

Texto completo
Autor(es):
Araujo Flores, Gabriela Venicia [1] ; Sandoval Pacheco, Carmen Maria [1] ; Tomokane, Thaise Yumie [1] ; Ochoa, Wilfredo Sosa [1, 2] ; Valeriano, Concepcion Zuniga [3] ; Castro Gomes, Claudia Maria [1] ; Pereira Corbett, Carlos Eduardo [1] ; Laurenti, Marcia Dalastra [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Lab Pathol Infect Dis, Sao Paulo, SP - Brazil
[2] Natl Autonomous Univ Honduras, Sch Microbiol, Tegucigalpa - Honduras
[3] Univ Sch Hosp, Tegucigalpa - Honduras
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Mediators of Inflammation; 2018.
Citações Web of Science: 0
Resumo

In Honduras, Leishmania (L.) infantum chagasi causes both visceral leishmaniasis (LV) and nonulcerated or atypical cutaneous leishmaniasis (NUCL). NUCL is characterized by mononuclear inflammatory infiltration of the dermis, composed mainly of lymphocytes followed by macrophages with discrete parasitism. Considering that little is known about the pathogenesis of NUCL, the aim of this study was to evaluate the regulatory response in situ in skin lesions of patients affected by NUCL. Biopsies (n = 20) from human cutaneous nonulcerative lesions were collected and processed by usual histological techniques. The in situ regulatory immune response was evaluated by immunohistochemistry using antihuman CD4, FoxP3, IL-10, and TGF-beta antibodies. CD4(+), FoxP3(+), TGF-beta(+), and IL-10(+) cells were observed in the dermis with inflammatory infiltration in all studied cases and at higher densities compared to the normal skin controls. A positive and strong correlation was observed between CD4(+) and FoxP3(+) cells, and a positive and moderate correlation was observed between FoxP3(+) and TGF-beta(+) but not with IL-10(+) cells. The data suggest that T regulatory FoxP3(+) cells and the regulatory cytokines, especially TGF-beta, play an important role in the immunopathogenesis of NUCL, modulating a cellular immune response in the skin, avoiding tissue damage, and leading to low tissue parasitic persistence. (AU)

Processo FAPESP: 15/01154-7 - Leishmaniose cutânea não ulcerada ou atípica causada pela Leishmania infantum chagasi no município de Amapala, Valle, Honduras: caracterização imuno-histopatológica das lesões de pele
Beneficiário:Carmen Maria Sandoval Pacheco
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 14/50315-0 - Leishmanioses na América Latina: uma perspectiva avançada sobre fatores imunopatogenéticos da infecção cutânea e visceral, imunomoduladores da saliva de vetores flebotomíneos e exo-antígenos imunogênicos de Leishmania (L.) infantum chagasi candidatos à vacina
Beneficiário:Carlos Eduardo Pereira Corbett
Linha de fomento: Auxílio à Pesquisa - Temático