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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Multiresidue Method for Quantification of Sulfonamides and Trimethoprim in Tilapia Fillet by Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry Using QuEChERS for Sample Preparation

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Autor(es):
Nunes, Katia S. D. [1] ; Assalin, Marcia R. [2] ; Vallim, Jose H. [2] ; Jonsson, Claudio M. [2] ; Queiroz, Sonia C. N. [2] ; Reyes, Felix G. R. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil
[2] Embrapa Meio Ambiente, POB 69, BR-13820000 Jaguariuna, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY; 2018.
Citações Web of Science: 4
Resumo

A multiresidue method for detecting and quantifying sulfonamides (sulfapyridine, sulfamerazine, sulfathiazole, sulfamethazine, sulfadimethoxine, sulfamethoxazole, and sulfamethoxypyridazine) and trimethoprim in tilapia 7 llet (Oreochromis niloticus) using liquid chromatography coupled to mass spectrometry was developed and validated.. e sample preparation was optimized using the QuEChERS approach.. e chromatographic separation was performed using a C18 column and 0.1% formic acid in water and acetonitrile as the mobile phase in the isocratic elution mode. Method validation was performed based on the Commission Decision 2002/657/EC and Brazilian guideline.. e validation parameters evaluated were linearity (r >= 0.99); limits of detection (LOD) and quanti 7 cation (LOQ), 1 ng center dot g(-1) and 5 ng center dot g(-1), respectively; intraday and interdays precision (CV lower than 19.4%).. e decision limit (CC alpha 102.6-120.0 ng center dot g(-1) and 70 ng center dot g(-1) for sulfonamides and trimethoprim, respectively) and detection capability (CC ss 111.7-140.1 ng center dot g(-1) and 89.9 ng center dot g(-1) for sulfonamides and trimethoprim, respectively) were determined. Analyses of tilapia 7 llet samples from 7 sh exposed to sulfamethazine through feed (incurred samples) were conducted in order to evaluate the method.. is new method was demonstrated to be fast, sensitive, and suitable for monitoring sulfonamides and trimethoprim in tilapia 7 llet in health surveillance programs, as well as to be used in pharmacokinetics and residue depletion studies. (AU)

Processo FAPESP: 13/50452-5 - Veterinary drugs: development and validation of bioanalytical methods using LC-MS/MS and MALDI-TOF-MS and its applications in sanitary surveillance and the development of fish farming in Brazil
Beneficiário:Felix Guillermo Reyes Reyes
Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE