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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging

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Autor(es):
Goncalves, Tatiana Maria [1] ; Garcia Martins, Regina Helena [1] ; Pessin Adriana, Bueno Benito [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Botucatu Med Sch, Dept Ophthalmol Otorhinolaryngol & Head Neck Surg, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF VOICE; v. 32, n. 1, p. 3-7, JAN 2018.
Citações Web of Science: 2
Resumo

Introduction. Microscopy studies of presbylarynx have identified epithelial atrophy, as well as vocal muscle atrophy, increased collagen, and decreased nonfibrous proteins of the extracellular matrix. Studies on the ultrastructure of presbylarynx are rare and can help us understand the pathophysiology of presbyphonia. Objective. To describe details on the ultrastructure of presbylarynx by transmission electron microscopy (TEM). Subjects and Methods. Sixteen human larynges were removed and distributed in two groups: control (n = 8; 30-50 years; six females, two males) and seniors (n = 8; 75-92 years; six females, two males). After preparation for TEM, the specimens, including epithelium and lamina propria, were examined and photographed at increasing magnification. Results. Control: the epithelium was intact, with overlapped cells, and with desmosomes between the intercellular junctions. The basement membrane was continuous and uniform, and the lamina propria contained collagen and elastic fibers in a regularly distributed loose arrangement, as well as some fibroblasts of different shapes. Senior: the epithelial cells were separated by enlarged intercellular junctions. The basement membrane was delicate and continuous. In the lamina propria there was predominance of elongated fibroblasts. Under the basement membrane a dense network of collagen fibers could be identified. Conclusions. In this study with TEM some structural changes were identified, particularly in the larynx of the elderly, both in the epithelium and lamina propria, some of them with likely participation of fibroblasts, which reinforces the importance of additional ultrastructure as well as molecular studies targeting those cells, as they are the main precursors of the components of the extracellular matrix. (AU)

Processo FAPESP: 14/13741-1 - Estudo de microscopia eletrônica de varredura e de transmissão das pregas vocais de idosos
Beneficiário:Regina Helena Garcia Martins
Linha de fomento: Auxílio à Pesquisa - Regular