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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Graves’ ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts

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Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ENDOCRINOLOGY METABOLISM; n. ahead, p. -, 2018.
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ABSTRACT Objective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). rRARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. (AU)

Processo FAPESP: 11/18464-8 - Expressão gênica de diferentes receptores hormonais em fibroblastos orbitais, submetidos à diferenciação adipocítica, de pacientes com oftalmopatia de graves
Beneficiário:Sarah Santiloni Cury
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 03/03651-0 - Estudo clínico, etiopatogênico e molecular da oftalmopatia de graves
Beneficiário:Gláucia Maria Ferreira da Silva Mazeto
Linha de fomento: Auxílio à Pesquisa - Regular