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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Widespread modulation of gene expression by copy number variation in skeletal muscle

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Autor(es):
Geistlinger, Ludwig [1, 2, 3, 4, 5] ; da Silva, Vinicius Henrique [2, 3] ; Mello Cesar, Aline Silva [2] ; Tizioto, Polyana Cristine [2] ; Waldron, Levi [4, 5] ; Zimmer, Ralf [3] ; de Almeida Regitano, Luciana Correia [1] ; Coutinho, Luiz Lehmann [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Embrapa Pecuaria Sudeste, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Luiz Queiroz Coll Agr ESALQ, Anim Sci Dept, Anim Biotechnol Lab, BR-13418900 Piracicaba - Brazil
[3] Ludwig Maximilians Univ Munchen LMU, Inst Bioinformat, Dept Informat, D-80333 Munich - Germany
[4] CUNY, Grad Sch Publ Hlth & Hlth Policy, New York, NY 10027 - USA
[5] CUNY, Inst Implementat Sci & Populat Hlth, New York, NY 10027 - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 8, JAN 23 2018.
Citações Web of Science: 2
Resumo

Copy number variation (CNV) is a frequently observed deviation from the diploid state due to duplication or deletion of genomic regions. Although intensively analyzed for association with diseases and production traits, the specific mechanisms and extent by which such variations affect the phenotype are incompletely understood. We present an integrative study on CNV and genome-wide gene expression in Brazilian Bos indicus cattle. We analyzed CNVs inferred from SNP-chip data for effects on gene expression measured with RNA-seq in skeletal muscle samples of 183 steers. Local effects, where expression changes coincided with CNVs in the respective genes, were restricted to immune genes. Distal effects were attributable to several high-impact CNVs that modulated remote expression in an orchestrated and intertwined fashion. These CNVs were located in the vicinity of major skeletal muscle pathway regulators and associated genes were enriched for proteolysis, autophagy, and muscle structure development. From association analysis between CNVs and several meat quality and production traits, we found CNV-associated expression effects to also manifest at the phenotype level. Based on genome sequences of the population founders, we further demonstrate that CNVs with impact on expression and phenotype are passed on from one generation to another. (AU)

Processo FAPESP: 12/23638-8 - Bases moleculares da qualidade da carne em bovinos da raça Nelore
Beneficiário:Luciana Correia de Almeida Regitano
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/01438-2 - Vias metabólicas e maciez da carne: de CNVs a expressão gênica em bovinos da raça Nelore
Beneficiário:Vinicius Henrique da Silva
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
Processo FAPESP: 16/11775-1 - Bases moleculares da qualidade de carne em bovinos da raça Nelore
Beneficiário:Ludwig Geistlinger
Linha de fomento: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico