Arginine and Polyamines Fate in Leishmania Infection

Autor(es)

Muxel, Sandra M. ^[1] ; Aoki, Juliana I. ^[1] ; Fernandes, Juliane C. R. ^[1] ; Laranjeira-Silva, Maria F. ^[1] ; Zampieri, Ricardo A. ^[1] ; Acuna, Stephanie M. ^[1] ; Muller, Karl E. ^[2] ; Vanderlinde, Rubia H. ^[1] ; Floeter-Winter, Lucile M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, Sao Paulo - Brazil

^[2] Univ Bergen, Dept Clin Sci, Bergen - Norway

• Tipo de documento: Artigo de Revisão
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 8, JAN 15 2018.
• Citações Web of Science: 12

Resumo

Leishmania is a protozoan parasite that alternates its life cycle between the sand fly and the mammalian host macrophages, involving several environmental changes. The parasite responds to these changes by promoting a rapid metabolic adaptation through cellular signaling modifications that lead to transcriptional and post-transcriptional gene expression regulation and morphological modifications. Molecular approaches such as gene expression regulation, next-generation sequencing (NGS), microRNA (miRNA) expression profiling, in cell Western blot analyses and enzymatic activity profiling, have been used to characterize the infection of murine BALB/c and C57BL/6 macrophages, as well as the human monocytic cell-lineage THP-1, with Leishmania amazonensis wild type (La-WT) or arginase knockout (La-arg(-)). These models are being used to elucidate physiological roles of arginine and polyamines pathways and the importance of arginase for the establishment of the infection. In this review, we will describe the main aspects of Leishmania-host interaction, focusing on the arginine and polyamines pathways and pointing to possible targets to be used for prognosis and/or in the control of the infection. The parasite enzymes, arginase and nitric oxide synthase-like, have essential roles in the parasite survival and in the maintenance of infection. On the other hand, in mammalian macrophages, defense mechanisms are activated inducing alterations in the mRNA, miRNA and enzymatic profiles that lead to the control of infection. Furthermore, the genetic background of both parasite and host are also important to define the fate of infection. (AU)

• Processo FAPESP: 16/22896-4 - Análise do perfil de miRNA de macrófagos humanos derivados da linhagem THP-1 e infectados por Leishmania (L.) amazonensis
• Beneficiário: Juliane Cristina Ribeiro Fernandes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/10461-3 - Papel do transportador de aminoácidos AAP3: expressão e purificação da proteína recombinante, obtenção do soro policlonal, localização da proteína em promastigotas de Leishmania amazonensis e teste de imunogenicidade em soro de animais infectados
• Beneficiário: Juliana Ide Aoki
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 14/50717-1 - Estudos bioquímicos, moleculares e funcionais da relação Leishmania-macrófago
• Beneficiário: Lucile Maria Floeter-Winter
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/25942-4 - Estabelecimento de perfil de miRNA de macrófagos murinos incfetados por Leishmania (L.) amazonensis
• Beneficiário: Stephanie Maia Acuna
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/19815-2 - Papel dos microRNAs na resposta imune mediada por TLRs na infecção por Leishmania amazonensis.
• Beneficiário: Sandra Marcia Muxel
• Modalidade de apoio: Auxílio à Pesquisa - Regular