Comprehensive untargeted metabolomics of Lychnnophorinae subtribe (Asteraceae: Vernonieae) in a phylogenetic context

Autor(es)

Poleti Martucci, Maria Elvira ^[1] ; Loeuille, Benoit ^[2] ; Pirani, Jose Rubens ^[2] ; Gobbo-Neto, Leonardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Inst Biosci, Dept Bot, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 1 JAN 11 2018.
• Citações Web of Science: 4

Resumo

Members of the subtribe Lychnophorinae occur mostly within the Cerrado domain of the Brazilian Central Plateau. The relationships between its 11 genera, as well as between Lychnophorinae and other subtribes belonging to the tribe Vernonieae, have recently been investigated upon a phylogeny based on molecular and morphological data. We report the use of a comprehensive untargeted metabolomics approach, combining HPLC-MS and GC-MS data, followed by multivariate analyses aiming to assess the congruence between metabolomics data and the phylogenetic hypothesis, as well as its potential as a chemotaxonomic tool. We analyzed 78 species by UHPLC-MS and GC-MS in both positive and negative ionization modes. The metabolic profiles obtained for these species were treated in MetAlign and in MSClust and the matrices generated were used in SIMCA for hierarchical cluster analyses, principal component analyses and orthogonal partial least square discriminant analysis. The results showed that metabolomic analyses are mostly congruent with the phylogenetic hypothesis especially at lower taxonomic levels (Lychnophora or Eremanthus). Our results confirm that data generated using metabolomics provide evidence for chemotaxonomical studies, especially for phylogenetic inference of the Lychnophorinae subtribe and insight into the evolution of the secondary metabolites of this group. (AU)

• Processo FAPESP: 12/16646-4 - Metabolômica e screening de interações ecoquímicas de plantas da subtribo Lychnophorinae (Asteraceae)
• Beneficiário: Maria Elvira Poleti Martucci
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/51454-3 - Estudos morfoanatômicos, metabolômicos e moleculares como subsídios à sistemática de espécies de Asteraceae e acesso ao seu potencial farmacológico
• Beneficiário: Beatriz Appezzato da Glória
• Modalidade de apoio: Auxílio à Pesquisa - Temático