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Distribution of Major Pilin Subunit Genes Among Atypical Enteropathogenic Escherichia coli and Influence of Growth Media on Expression of the ecp Operon

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Autor(es):
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Munhoz, Danielle D. [1] ; Nara, Julia M. [1, 2] ; Freitas, Natalia C. [1] ; Moraes, Claudia T. P. [1] ; Nunes, Kamila O. [1] ; Yamamoto, Bruno B. [1] ; Vasconcellos, Francielli M. [1] ; Martinez-Laguna, Ygnacio [3] ; Giron, Jorge A. [3] ; Martins, Fernando H. [1] ; Abe, Cecilia M. [1] ; Elias, Waldir P. [1] ; Piazza, Roxane M. F. [1]
Número total de Autores: 13
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil
[2] Inst Biol, Lab Doencas Suinos Washington Sugay, Sao Paulo - Brazil
[3] Benemerita Univ Autonoma Puebla, Ctr Detecc Biomol, Puebla - Mexico
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 9, MAY 15 2018.
Citações Web of Science: 0
Resumo

Atypical enteropathogenic Escherichia coil (aEPEC) strains are unable to produce the bundle-forming pilus (BFP), which is responsible for the localized adherence pattern, a characteristic of the pathogenicity of typical EPEC strains. The lack of BFP in aEPEC strains suggests that other fimbrial or non-fimbrial adhesins are involved in their adhesion to the host cells. The aim of this study was to investigate the distribution of major subunit fimbrial genes known to be important adherence factors produced by several E. coil pathotypes in a collection of 72 aEPEC strains. Our results demonstrate that a high percentage (94-100%) of aEPEC strains harbored ecpA, fimA, hcpA, and lpfA fimbrial genes. Other fimbrial genes including pilS, pilV, sfpA, daaC, papA, and sfa were detected at lower frequencies (1-8%). Genes encoding fimbrial subunits, which are characteristic of enteroaggregative E. coli or enterotoxigenic E. coli were not found. No correlation was found between fimbrial gene profiles and adherence phenotypes. Since all aEPEC strains contained ecpA, the major pilin gene of the E. coil common pilus (ECP), a subset of ecpA+ strains was analyzed for transcription of ecpRABCDE and production of ECP upon growth in three different culture conditions at 37 degrees C. Transcription of ecpRABCDE occurred in all conditions; however, ECP production was medium dependent. In all, the data suggest that aEPEC strains are highly heterogeneous in terms of their fimbrial gene profiles. Despite lacking BFP production, other mechanisms of cell adherence exist in aEPEC strains to ensure host colonization, e.g., mediated by other prevalent pili such as ECP. Moreover, the production of ECP by aEPEC strains might be influenced by yet unknown post-transcriptional factors. (AU)

Processo FAPESP: 09/14845-7 - Imunodiagnóstico de Escherichia coli diarreiogênica
Beneficiário:Roxane Maria Fontes Piazza
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/06589-6 - Teste imunocromatográfico como ferramenta de diagnóstico de doenças negligenciadas: estudos de aprimoramento, escalonamento e viabilidade
Beneficiário:Roxane Maria Fontes Piazza
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 04/12136-5 - Escherichia coli enteropatogênica atípica (EPEC atípica)
Beneficiário:Waldir Pereira Elias Junior
Linha de fomento: Auxílio à Pesquisa - Temático