Fingerprinting metabolomics in tropical mistletoes: A case study with facultative aluminum-accumulating species

Autor(es)

de Souza, Marcelo Claro ^{[1, 2]} ; Rosa, Annylory Lima ^[1] ; Poschenrieder, Charlotte ^[3] ; Tolra, Roser ^[3] ; Da Costa, Fernando Batista ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, AsterBioChem Res Team, Ave Cafe S-N, BR-14040020 Ribeirao Preto, SP - Brazil

^[2] Univ Brasilia, Dept Bot, Inst Biol Sci, Campus Univ Darcy Ribeiro, BR-70910900 Brasilia, DF - Brazil

^[3] Autonomous Univ Barcelona, Bellaterra - Spain

• Tipo de documento: Artigo Científico
• Fonte: PHYTOCHEMISTRY LETTERS; v. 25, p. 90-94, JUN 2018.
• Citações Web of Science: 2

Resumo

Aluminum (Al) toxicity is a hot topic due to the high sensitivity of cultivated plants. Despite the large investment in the last century in understanding the mechanisms used by sensitive and Al-excluding species to avoid Al uptake from soil, little attention has been devoted to understanding the mechanisms used by native Al-accumulating species to deal with Al-toxicity. Passovia ovata (Pohl ex DC.) Kuijt and Struthanthus polyanthus Mart. are mistletoes with a facultative Al-accumulating behavior. In the Brazilian Cerrado they are commonly found infecting Al-accumulating (Miconia albicans (Sw.) Steud.) and non-accumulating (Byrsonima verbascifolia (L.) DC.) species. Taking into account the importance of organic complexes in the detoxification of the highly toxic Al3+ ions, it is to be expected that mistletoes differ in their metabolomic profile when feeding on species differing in Al accumulation. Here we tested this hypothesis using an untargeted LC-MS approach to investigate the influence of Al on the metabolome of P. ovata and S. polyanthus infecting M. albicans and B. verbascifolia under field conditions. We observed differences in the metabolic profiles between mistletoes growing on Al-accumulating and Al-excluding hosts, and also observed a positive correlation between Al leaf-accumulation and the metabolic profile. Using the OPLS-DA, we identified quinic acid (phenolic compound) as a metabolic biomarker distinguishing mistletoes grown under high and low Al availability. (AU)

• Processo FAPESP: 13/21293-6 - Avanços nos estudos metabolômico de espécies vegetais do cerrado em desenvolvimento sobre diferentes disponibilidades de alumínio
• Beneficiário: Marcelo Claro de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/26866-7 - Metabolômica, alvos enzimáticos e ferramentas in silico na busca por substâncias bioativas de plantas
• Beneficiário: Fernando Batista da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51454-3 - Estudos morfoanatômicos, metabolômicos e moleculares como subsídios à sistemática de espécies de Asteraceae e acesso ao seu potencial farmacológico
• Beneficiário: Beatriz Appezzato da Glória
• Modalidade de apoio: Auxílio à Pesquisa - Temático