[1, 2, 3]
Kakimoto, Pamela A.
Kowaltowski, Alicia J.
Número total de Autores: 5
Afiliação do(s) autor(es):
 UCLA, David Geffen Sch Med, Dept Med, Div Endocrinol Diabet & Hypertens, Los Angeles, CA 90095 - USA
 Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 - USA
 Kuwait Univ, Dept Physiol, Fac Med, Kuwait - Kuwait
 Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento:
Artigo de Revisão
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS;
Citações Web of Science:
High plasma levels of fatty acids occur in a variety of metabolic diseases. Cellular effects of fatty acid overload resulting in negative cellular responses (lipotoxicity) are often studied in vitro, in an attempt to understand mechanisms involved in these diseases. Fatty acids are poorly soluble, and thus usually studied when complexed to albumins such as bovine serum albumin (BSA). The conjugation of fatty acids to albumin requires care pertaining to preparation of the solutions, effective free fatty acid concentrations, use of different fatty acid species, types of BSA, appropriate controls and ensuring cellular fatty acid uptake. This review discusses lipotoxicity models, the potential problems encountered when using these cellular models, as well as practical solutions for difficulties encountered. (AU)