Preeclamptic plasma stimulates the expression of m... - BV FAPESP
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Preeclamptic plasma stimulates the expression of miRNAs, leading to a decrease in endothelin-1 production in endothelial cells

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Autor(es):
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Caldeira-Dias, Mayara [1] ; Luizon, Marcelo R. [1] ; Deffune, Elenice [2] ; Tanus-Santos, Jose E. [3] ; Freire, Paula P. [4] ; Carvalho, Robson F. [4] ; Bettiol, Heloisa [5] ; Cardoso, Viviane C. [5] ; Barbieri, Marco Antonio [5] ; Cavalli, Ricardo C. [6] ; Sandrim, Valeria C. [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Pharmacol, BR-18618000 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Cell Engn Lab, Blood Transfus Ctr, Botucatu Med Sch, Botucatu, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, SP - Brazil
[4] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Morphol, Botucatu, SP - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH; v. 12, p. 75-81, APR 2018.
Citações Web of Science: 3
Resumo

Preeclampsia is a major cause of maternal and fetal morbidity and mortality worldwide. It is a multisystem pregnancy syndrome characterized by general endothelial dysfunction caused mainly by plasma factors and debris in endothelial cells. It is widely accepted that endothelin-1 (ET-1) is involved in the pathophysiology of preeclampsia, and so it is of interest to ascertain whether the ET-1 gene (EDN1) can be targeted with tools such as miRNAs. Therefore, we investigated the relationship between the expression of miRNAs that putatively target EDN1 (and so affect ET-1 levels) in HUVECs incubated with plasma from preeclamptic women. EDN1 expression and ET-1 levels in HUVECs incubated with plasma from women with preeclampsia were similar to those in plasma from healthy pregnant women. Expression of miRNAs let-7a, -7b, and -7c, and to a lesser degree 125a and 125b, was increased in preeclampsia. Expression of miRNAs of the let-7 family was significantly negatively correlated with ET-1 levels in preeclampsia. Transfection of the preeclampsia cultures with mimic miRNA let-7 decreased ET-1 levels. Our findings show that preeclamptic plasma stimulates the expression of miRNAs in HUVECs, leading to a decrease in ET-1levels, which suggests that therapeutic miRNAs may aid in the management of preeclampsia. (AU)

Processo FAPESP: 13/12919-9 - MicroRNAs circulantes em pré-eclâmpsia
Beneficiário:Valeria Cristina Sandrim
Modalidade de apoio: Auxílio à Pesquisa - Regular