| Texto completo | |
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Caldeira-Dias, Mayara
[1]
;
Luizon, Marcelo R.
[1]
;
Deffune, Elenice
[2]
;
Tanus-Santos, Jose E.
[3]
;
Freire, Paula P.
[4]
;
Carvalho, Robson F.
[4]
;
Bettiol, Heloisa
[5]
;
Cardoso, Viviane C.
[5]
;
Barbieri, Marco Antonio
[5]
;
Cavalli, Ricardo C.
[6]
;
Sandrim, Valeria C.
[1]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Pharmacol, BR-18618000 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Cell Engn Lab, Blood Transfus Ctr, Botucatu Med Sch, Botucatu, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, SP - Brazil
[4] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Morphol, Botucatu, SP - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH; v. 12, p. 75-81, APR 2018. |
| Citações Web of Science: | 3 |
| Resumo | |
Preeclampsia is a major cause of maternal and fetal morbidity and mortality worldwide. It is a multisystem pregnancy syndrome characterized by general endothelial dysfunction caused mainly by plasma factors and debris in endothelial cells. It is widely accepted that endothelin-1 (ET-1) is involved in the pathophysiology of preeclampsia, and so it is of interest to ascertain whether the ET-1 gene (EDN1) can be targeted with tools such as miRNAs. Therefore, we investigated the relationship between the expression of miRNAs that putatively target EDN1 (and so affect ET-1 levels) in HUVECs incubated with plasma from preeclamptic women. EDN1 expression and ET-1 levels in HUVECs incubated with plasma from women with preeclampsia were similar to those in plasma from healthy pregnant women. Expression of miRNAs let-7a, -7b, and -7c, and to a lesser degree 125a and 125b, was increased in preeclampsia. Expression of miRNAs of the let-7 family was significantly negatively correlated with ET-1 levels in preeclampsia. Transfection of the preeclampsia cultures with mimic miRNA let-7 decreased ET-1 levels. Our findings show that preeclamptic plasma stimulates the expression of miRNAs in HUVECs, leading to a decrease in ET-1levels, which suggests that therapeutic miRNAs may aid in the management of preeclampsia. (AU) | |
| Processo FAPESP: | 13/12919-9 - MicroRNAs circulantes em pré-eclâmpsia |
| Beneficiário: | Valeria Cristina Sandrim |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |