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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Microexon gene transcriptional profiles and evolution provide insights into blood processing by the Schistosoma japonicum esophagus

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Li, Xiao-Hong [1] ; DeMarco, Ricardo [2] ; Neves, Leandro X. [3] ; James, Sally R. [4] ; Newling, Katherine [4] ; Ashton, Peter D. [4] ; Cao, Jian-Ping [1] ; Wilson, R. Alan [5] ; Castro-Borges, William [3]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai - Peoples R China
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil
[3] Univ Fed Ouro Preto, Dept Ciencias Biol, Campus Morro do Cruzeiro, Ouro Preto, MG - Brazil
[4] Univ York, Dept Biol, Genom & Bioinformat Lab, York, N Yorkshire - England
[5] Univ York, Dept Biol, Ctr Immunol & Infect, York, N Yorkshire - England
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: PLoS Neglected Tropical Diseases; v. 12, n. 2 FEB 2018.
Citações Web of Science: 2

Background Adult schistosomes have a well-developed alimentary tract comprising an oral sucker around the mouth, a short esophagus and a blind ending gut. The esophagus is not simply a muscular tube for conducting blood from the mouth to gut but is divided into compartments, surrounded by anterior and posterior glands, where processing of ingested blood is initiated. Self-cure of rhesus macaques from a Schistosoma japonicum infection appears to operate by blocking the secretory functions of these glands so that the worms cease feeding and slowly starve to death. Here we use subtractive RNASeq to characterise the genes encoding the principal secretory products of S. japonicum esophageal glands, preparatory to evaluating their relevance as targets of the self-cure process. Methodology/Principal findings The heads and a small portion of the rear end of male and female S. japonicum worms were separately enriched by microdissection, for mRNA isolation and library construction. The sequence reads were then assembled de novo using Trinity and those genes enriched more than eightfold in the head preparation were subjected to detailed bioinformatics analysis. Of the 62 genes selected from the male heads, more than one third comprised MEGs encoding secreted or membrane-anchored proteins. Database searching using conserved motifs revealed that the MEG-4 and MEG-8/9 families had counterparts in the bird schistosome Tri-chobilharzia regenti, indicating an ancient association with blood processing. A second group of MEGs, including a MEG-26 family, encoded short peptides with amphipathic properties that most likely interact with ingested host cell membranes to destabilise them. A number of lysosomal hydrolases, two protease inhibitors, a secreted VAL and a putative natterin complete the line-up. There was surprisingly little difference between expression patterns in males and females despite the latter processing much more blood. Significance/Conclusions The mixture of approximately 40 proteins specifically secreted by the esophageal glands is responsible for initiating blood processing in the adult worm esophagus. They comprise the potential targets for the self-cure process in the rhesus macaque, and thus represent a completely new cohort of secreted proteins that can be investigated as vaccine candidates. (AU)

Processo FAPESP: 14/09361-9 - Estudo dos genes de micro-exon (MEGs) do parasita humano Schistosoma mansoni e da interação de seus produtos protéicos com células humanas
Beneficiário:Ricardo de Marco
Linha de fomento: Auxílio à Pesquisa - Regular