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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Long-term oral exposure to safe dose of bisphenol A in association with high-fat diet stimulate the prostatic lesions in a rodent model for prostate cancer

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Autor(es):
Facina, Camila H. [1] ; Campos, Silvana G. P. [1] ; Goncalves, Bianca F. [1] ; Goes, Rejane M. [1, 2] ; Vilamaior, Patricia S. L. [1] ; Taboga, Sebastiao R. [1, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas IBILCE, Lab Microscopia & Microanal, Sao Paulo - Brazil
[2] Univ Estadual Campinas UNICAMP, Dept Biol Estrutural & Func, Inst Biol, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PROSTATE; v. 78, n. 2, p. 152-163, FEB 1 2018.
Citações Web of Science: 6
Resumo

BackgroundStudies have shown that exposure to environmental chemicals known as endocrine disruptors can cause permanent changes in genital organs, such as the prostate. Among these environmental chemicals stands out bisphenol A (BPA). Another factor associated with prostate changes is the consumption of a high-fat diet. Although the relationship between the consumption of a high-fat diet and an increased risk of prostate cancer is well established, the mechanisms that lead to the establishment of this disease are not completely understood, nor the simultaneous action of BPA and high-fat diet. MethodsAdult gerbils (100 days old) were divided in four groups (n=6 per group): Control (C): animals that received a control diet and filtered water; Diet (D): animals that received a high-fat diet and filtered water; BPA: animals that received a control diet and BPA - 50 mu gkg(-1) day(-1) in drinking water; BPA+Diet (BPA+D): animals that received a high-fat diet+BPA - 50 mu gkg(-1) day(-1) in drinking water. After the experimental period (6 months), the dorsolateral and ventral prostate lobes were removed, and analyzed by several methods. ResultsHistological analysis indicated premalignant and malignant lesions in both prostatic lobes. However, animals of the D, BPA, and BPA+D groups showed a higher incidence and larger number of prostatic lesions; inflammatory foci were also common. Markers to assess prostate lesions, such as increased activation of the DNA repair system (PCNA-positive cells), androgen receptor (AR), and number of basal cells, confirmed the histology. However, serum levels of testosterone did not change under the experimental conditions. ConclusionsThe results indicated that the methodology used was effective in generating metabolic changes, which directly compromised prostatic homeostasis. Diet and BPA appear to modulate the activation of the AR pathway and thereby optimize tumor establishment in the gerbil prostate. (AU)

Processo FAPESP: 13/01619-4 - Ação do desregulador endócrino bisfenol a e da dieta hiperlipídica sobre os lobos prostáticos do gerbilo
Beneficiário:Camila Helena Facina
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/53990-2 - EMU: aquisição de microscópio de varredura confocal a laser para um centro multiusuário de microscopia da UNESP
Beneficiário:Sebastião Roberto Taboga
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários