| Texto completo | |
| Autor(es): |
Soares, Diana G.
[1]
;
Anovazzi, Giovanna
[2]
;
Bordini, Ester Alves F.
[3]
;
Zuta, Uxua O.
[3]
;
Silva Leite, Maria Luisa A.
[3]
;
Basso, Fernanda G.
[3]
;
Hebling, Josimeri
[2]
;
de Souza Costa, Carlos A.
[3]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Baum Sch Dent, Dept Operat Dent Endondont & Dent Mat, Sao Paulo - Brazil
[2] Univ Estadual Paulista, UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, Sao Paulo - Brazil
[3] Univ Estadual Paulista, UNESP, Dept Physiol & Pathol, Araraquara Sch Dent, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF ENDODONTICS; v. 44, n. 6, p. 971-976, JUN 2018. |
| Citações Web of Science: | 2 |
| Resumo | |
Introduction: The improvement of biomaterials capable of driving the regeneration of the pulp-dentin complex mediated by resident cells is the goal of regenerative dentistry. In the present investigation, a chitosan scaffold (CHSC) that released bioactive concentrations of simvastatin (SIM) was tested, aimed at the development of a cell-free tissue engineering system. Methods: First, we performed a dose-response assay to select the bioactive dose of SIM capable of inducing an odontoblastic phenotype in dental pulp cells (DPCs); after which we evaluated the synergistic effect of this dosage with the CHSC/DPC construct. SIM at 1.0 mu mol/L (CHSC-SIM1.0) and 0.5 mu mol/L were incorporated into the CHSC, and cell viability, adhesion, and calcium deposition were evaluated. Finally, we assessed the biomaterials in an artificial pulp chamber/3-dimensional culture model to simulate the cell-free approach in vitro. Results: SIM at 0.1 Amol/L was selected as the bioactive dose. This drug was capable of strongly inducing an odontoblastic phenotype on the DPC/CHSC construct. The incorporation of SIM into CHSC had no deleterious effect on cell viability and adhesion to the scaffold structure. CHSC-SIM1.0 led to significantly higher calcium-rich matrix deposition on scaffold/dentin disc assay compared with the control (CHSC). This biomaterial induced the migration of DPCs from a 3-dimensional culture to its surface as well as stimulated significantly higher expressions of alkaline phosphatase, collagen type 1 alpha 1, dentin matrix acidic phosphoprotein 1, and dentin sialophosphoprotein on 3-dimensional cultured DPCs than on those in contact with CHSC. Conclusions: CHSC-SIM1.0 scaffold was capable of increasing the chemotaxis and regenerative potential of DPCs. (AU) | |
| Processo FAPESP: | 13/23520-0 - Bioatividade de scaffolds experimentais a base de quitosana e colágeno sobre cultura de células pulpares humanas |
| Beneficiário: | Diana Gabriela Soares dos Passos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/15674-5 - Associação de técnicas de engenharia tecidual para modulação da regeneração de tecidos mineralizados sob inflamação degenerativa: análise em modelos de cultura-3D em biorreator de perfusão e inflamatórios em animais |
| Beneficiário: | Diana Gabriela Soares dos Passos |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |