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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Metabolic responses of Eucalyptus species to different temperature regimes

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Autor(es):
Mokochinski, Joao Benhur [1, 2] ; Mazzafera, Paulo [1] ; Helena Frankland Sawaya, Alexandra Christine [1, 3] ; Mumm, Roland [2] ; Hendricus de Vos, Ric Cornelis [2] ; Hall, Robert David [2, 4]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Plant Biol, BR-13083862 Campinas, SP - Brazil
[2] Wageningen Univ & Res, Wageningen Plant Res, Biosci, Wageningen - Netherlands
[3] Univ Estadual Campinas, UNICAMP, Fac Pharmaceut Sci, BR-13083862 Campinas, SP - Brazil
[4] Wageningen Univ & Res, Lab Plant Physiol, Wageningen - Netherlands
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF INTEGRATIVE PLANT BIOLOGY; v. 60, n. 5, p. 397-411, MAY 2018.
Citações Web of Science: 5
Resumo

Species and hybrids of Eucalyptus are the world's most widely planted hardwood trees. They are cultivated across a wide range of latitudes and therefore environmental conditions. In this context, comprehensive metabolomics approaches have been used to assess how different temperature regimes may affect the metabolism of three species of Eucalyptus, E. dunnii, E. grandis and E. pellita. Young plants were grown for 53 d in the greenhouse and then transferred to growth chambers at 10 degrees C, 20 degrees C or 30 degrees C for another 7 d. In all three species the leaf chlorophyll content was positively correlated to temperature, and in E. pellita the highest temperature also resulted in a significant increase in stem biomass. Comprehensive metabolomics was performed using untargeted gas chromatography mass spectrometry (GC-MS) and liquid chromatography (LC)-MS. This approach enabled the comparison of the relative abundance of 88 polar primary metabolites from GC-MS and 625 semi-polar secondary metabolites from LC-MS. Using principal components analysis, a major effect of temperature was observed in each species which was larger than that resulting from the genetic background. Compounds mostly affected by temperature treatment were subsequently selected using partial least squares discriminant analysis and were further identified. These putative annotations indicated that soluble sugars and several polyphenols, including tannins, triterpenes and alkaloids were mostly influenced. (AU)

Processo FAPESP: 13/21306-0 - Desenvolvimento de métodos analíticos por UPLC-MS para o estudo do metaboloma de duas espécies de eucalipto expostas a variações de temperatura e concentração de CO2
Beneficiário:João Benhur Mokochinski
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/51949-5 - Physiological responses of Eucalyptus globulus and E. grandis to high concentration of CO2 and temperature variations, identified by metabolomic and transcriptomic analyses
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
Processo FAPESP: 15/06987-7 - Análise metabolômica untargeted do eucalipto: da aquisição dos dados à interpretação biológica
Beneficiário:João Benhur Mokochinski
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático