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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Graves' ophthalmopathy: low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts

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Autor(es):
Cury, Sarah Santiloni [1] ; Oliveira, Miriane [1] ; Sibio, Maria Teresa [1] ; Clara, Sueli [1] ; Melo Luvizotto, Renata De Azevedo [1] ; Conde, Sandro [2] ; Jorge, Edson Nacib [3] ; Nunes, Vania dos Santos [1] ; Nogueira, Celia Regina [1] ; Ferreira da Silva Mazeto, Glaucia Maria [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Estadual Sao Paulo Unesp, Fac Med Botucatu, Dept Med Interna, Div Endocrinol, Botucatu, SP - Brazil
[2] Inst Fed Educ Ciencia & Tecnol Estado Sao Paulo I, Sao Roque, SP - Brazil
[3] Univ Estadual Sao Paulo Unesp, Fac Med Botucatu, Dept Oftalmol Otorrinolaringol & Cirurgia Cabeca, Botucatu, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ENDOCRINOLOGY METABOLISM; v. 62, n. 3, p. 366-369, MAY-JUN 2018.
Citações Web of Science: 0
Resumo

Objective: Graves' ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RAR alpha) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RAR alpha gene expression in the treated and untreated cells was then compared. Result: Fibroblast RAR alpha expression was not affected by 100 nM Dex On the other hand, RAR alpha expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusion: Orbital fibroblasts from a GO patient expressed the RAR alpha gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. (AU)

Processo FAPESP: 11/18464-8 - Expressão gênica de diferentes receptores hormonais em fibroblastos orbitais, submetidos à diferenciação adipocítica, de pacientes com oftalmopatia de graves
Beneficiário:Sarah Santiloni Cury
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 03/03651-0 - Estudo clínico, etiopatogênico e molecular da oftalmopatia de graves
Beneficiário:Gláucia Maria Ferreira da Silva Mazeto
Linha de fomento: Auxílio à Pesquisa - Regular