Congenital tremor in piglets: Is bovine viral diarrhea virus an etiological cause?

Autor(es)

Mechler, Marina Lopes ^[1] ; Gomes, Felipe dos Santos ^[1] ; Nascimento, Karla Alvarenga ^[1] ; de Souza-Pollo, Andressa ^[1] ; Barbosa Pires, Felipe Ferreira ^[1] ; Samara, Samir Issa ^[1] ; Pituco, Edviges Maristela ^[2] ; de Oliveira, Luis Guilherme ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci FCAV, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] Biol Inst Sao Paulo, Av Conselheiro Rodrigues Alves, 1252 Vila Mariana, BR-04014002 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 220, p. 107-112, JUL 2018.
• Citações Web of Science: 2

Resumo

Congenital tremor in pigs involves several etiologies, including pestivirus, which may cause neurological injuries in different animal species. To evaluate whether bovine viral diarrhea virus (BVDV), an important pestivirus, is one of the etiological agents of congenital tremor in swine, gilts and the fetuses were challenged at 45 days of gestation with BVDV-2. Four pregnant gilts were inoculated oronasally, four gilts underwent fetal intrauterine inoculation, and two gilts constituted the control group. Antibody titers were determined by virus neutralization (VN), and viral RNA was detected by RT-PCR. Blood samples were collected from all gilts and piglets born to obtain whole blood and serum for analysis. One third of the neonates were euthanized at three days old, and samples of the encephalon, brain stem and spinal cord were collected for anatomopathological evaluation and viral RNA detection. The piglets that remained alive were clinically evaluated every day, and blood sampling was performed regularly for 35 days. The piglets from gilts in both inoculation treatment groups showed no clinical neurological signs and were born with no viral RNA in their blood and organs. Piglets born from oronasally inoculated gilts did not present antibodies against BVDV-2 at birth, although they were acquired by passive maternal transfer. In contrast, intrauterine-inoculated piglets were born with high antibody titers (80 to 640) against the agent, which remained high until the end of the experimental period. Microscopically, no noticeable changes were observed. Macroscopically, 29.5% of the total piglets euthanized, from both inoculation groups, were born with a low cerebellar:brain ratio. Nevertheless, some piglets had a high cerebellar:brain ratio, indicating the need for standardizing this value. Thus, it was concluded that BVDV is not an etiological agent for congenital swine tremor. (AU)

• Processo FAPESP: 16/21421-2 - Patogenia da infecção pelo vírus da diarréia viral bovina em suínos: aspectos reprodutivos, neonatais e neurológicos
• Beneficiário: Luís Guilherme de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/02982-3 - Mioclonia congênita em suínos: vírus da diarreia viral bovina é um agente etiológico?
• Beneficiário: Marina Lopes Mechler Dreibi
• Modalidade de apoio: Bolsas no Brasil - Mestrado