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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Eucalyptus xylan: An in-house-produced substrate for xylanase evaluation to substitute birchwood xylan

Texto completo
Autor(es):
Corradini, Felipe A. S. [1] ; Baldez, Thais O. [1] ; Milessi, Thais S. S. [1, 2] ; Tardioli, Paulo W. [1, 2] ; Ferreira, Antonio G. [3] ; Giordano, Roberto de Campos [1, 2] ; Giordano, Raquel de L. C. [1, 2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Grad Program Chem Engn, POB 676, Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Chem Engn Dept, POB 676, Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Chem Dept, POB 676, Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Carbohydrate Polymers; v. 197, p. 167-173, OCT 1 2018.
Citações Web of Science: 2
Resumo

Over the past decades, most studies with xylanases have used Birchwood xylan as the standard substrate for activity assays. However, recently, Birchwood xylan production was discontinued by major suppliers, creating an important demand for a substitute. Ongoing and future studies require a substrate with characteristics equivalent to the discontinued xylan, in order to enable the comparison of results. In this context, a protocol for the production of a substrate similar to the discontinued commercial Birchwood xylan is reported. Obtained from bleached Eucalyptus cellulose pulp, xylan was extracted using 4% w/v NaOH solution at 25 degrees C, precipitated with glacial acetic acid (HOAc), and freeze-dried. A thermal pretreatment in an autoclave for 15 min increased its solubility. The resulting xylan was characterized by infrared spectroscopy, thermogravimetry, and NMR. When assessing the activity of xylanases, the results were the same as those for commercial Birchwood xylan. (AU)

Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
Beneficiário:Roberto de Campos Giordano
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático