Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Beneficial Role of Low-Intensity Laser Irradiation on Neural beta-tubulin III Protein Expression in Human Bone Marrow Multipotent Mesenchymal Stromal Cells

Texto completo
Autor(es):
Ferreira-Silva, Valeria [1, 2, 3] ; Primo, Fernando L. [4] ; Baqui, Munira M. A. [5] ; Magalhaes, Danielle A. R. [1, 2] ; Orellana, Maristela D. [1, 2] ; Castilho-Fernandes, Andrielle [3] ; Cruz, Mario C. [6] ; Camara, Niehls O. S. [6] ; Covas, Dimas T. [1, 2, 7] ; Tedesco, Antonio C. [3]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ctr Cell Therapy, BR-14051140 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Sch Med, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Reg Blood Ctr, BR-14051140 Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photobiol & Photomed Res Grp, Dept Chem, Ctr Nanotechnol & Tissue Engn, BR-14040901 Ribeirao Preto - Brazil
[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, BR-14800903 Araraquara, SP - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Cell & Mol Biol & Pathogen Bioagents, BR-14049900 Ribeirao Preto - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508900 Sao Paulo - Brazil
[7] Univ Sao Paulo, Sch Med, Dept Clin Med, BR-14048900 Ribeirao Preto - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: STEM CELL REVIEWS AND REPORTS; v. 14, n. 4, p. 585-598, AUG 2018.
Citações Web of Science: 1
Resumo

The purpose of the present study was to evaluate the neural protein expression pattern of human multipotent mesenchymal stromal cells (hMSCs) treated with forskolin (free-form/FF). The study investigated forskolin's capacity to enhance intracellular levels of cyclic adenosine monophosphate (cAMP) by activating adenylate cyclase and probably by inducing neuron-like cells in vitro. In addition, because nanotechnology is a growing field of tissue engineering, we also assessed the action of a new system called the nanostructured-forskolin (NF) to examine the improvement of drug delivery. Afterwards, the cells were submitted to low-level laser irradiation to evaluate possible photobiostimulatory effects. Investigations using the immunofluorescence by confocal microscopy and Western blot methods revealed the expression of the neuronal marker beta-tubulin III. Fluorescence intensity quantification analysis using INCell Analyzer System for beta-tubulin III was used to examine significant differences. The results showed that after low-level laser irradiation exposure, there was a tendency to increase the beta-tubulin III expression in all groups, as expected in the photobiostimulation process. Notably, this process induced for irradiation was more pronounced in irradiated nanoforskolin cells (INF) compared to non-irradiated free-forskolin control cells (NFFC). However, there was also an increase in beta-tubulin III protein expression in the groups: irradiated nanocontrol cells (INC) compared to non-irradiated free-forskolin control cells (NFF) and after treatment with non-irradiated free-forskolin (NFF) and non-irradiated nanoforskolin (NNFC). We concluded that the methods using low-level laser irradiation and/or nanoparticles showed an up-regulation of neural-protein expression in hMSCs that could be used to facilitate cellular therapy protocols in the near future. (AU)

Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático