aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine

Autor(es)

Garcia, Valdir Gouveia ^[1] ; Gualberto Junior, Erivan Clementino ^[1] ; Ervolino, Edilson ^{[1, 2]} ; Hitomi Nagata, Maria Jose ^[1] ; de Almeida, Juliano Milanezi ^[1] ; Theodoro, Leticia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Grp Res & Study Laser Dent, Dept Surg & Integrated Clin, Div Periodont, Sch Dent, 1193 Jose Bonifacio, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Basic Sci, Sch Dent, 1193 Jose Bonifacio, BR-16015050 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 22, p. 70-78, JUN 2018.
• Citações Web of Science: 6

Resumo

Background-This study evaluated the antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planning (SRP) for the treatment of experimental periodontitis (EP) in ovariectomized rats under systemic nicotine. Methods: Female ovariectomized rats (n = 180) were divided into two groups: vehicle administration (Veh) and nicotine administration (Nic). Mini-pumps containing either vehicle or nicotine were inserted in the rats 30 days before the induction of EP, which was induced by placing a ligature around the left mandibular first molar. The rats were randomly divided into three treatment subgroups: SRP, SRP plus low-level laser therapy (LLLT), and SRP plus aPDT. aPDT consisted of the application of a phenothiazine photosensitizer followed by LLLT. Ten animals from each group were euthanized at days 7, 15, and 30 after periodontal treatment. The furcation region was evaluated using histological, histometric analyses and immunolabelling for PCNA, TRAP, RANKL, and OPG. Results: Nicotine administration resulted in greater bone loss (BL). aPDT resulted in lower BL compared to SRP. aPDT showed higher quantities of PCNA-positive cells compared to SRP, regardless of the nicotine status. aPDT resulted in less recruitment of osteoclasts and lower RANKL immunolabelling compared to LLLT and SRP. Conclusion: aPDT was effective in animals treated with nicotine. (AU)

• Processo FAPESP: 10/15094-2 - Efeitos do laser e da terapia fotodinâmica no tratamento periodontal mecânico em ratas ovariectomizadas e sob influência sistêmica de nicotina: estudo histomorfométrico e imunoistoquímico
• Beneficiário: Erivan Clementino Gualberto Júnior
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/00936-0 - Efeitos do laser e da terapia fotodinâmica no tratamento periodontal mecânico em ratas ovariectomizadas e sob influência sistêmica de nicotina: estudo histomorfométrico e imunoistoquímico
• Beneficiário: Maria José Hitomi Nagata
• Modalidade de apoio: Auxílio à Pesquisa - Regular