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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Layer-by-layer hollow photosensitizer microcapsule design via a manganese carbonate hard template for photodynamic therapy in cells

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Autor(es):
Simioni, Andreza Ribeiro [1] ; Carvalho de Jesus, Priscila Costa [2, 3] ; Tedesco, Antonio Claudio [2, 3]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Vale do Paraiba Univ, Res & Dev Inst IPD, Organ Synth Lab, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos - Brazil
[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn Photobiol, Dept Chem, Av Bandeirantes 3900, BR-14040901 Vila Monte Alegre - Brazil
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photomed Res Grp, Av Bandeirantes 3900, BR-14040901 Vila Monte Alegre - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Photodiagnosis and Photodynamic Therapy; v. 22, p. 169-177, JUN 2018.
Citações Web of Science: 3
Resumo

Background: Microcapsules fabricated using layer-by-layer self-assembly have unique properties, making them attractive for drug delivery applications. The technique has been improved, allowing the deposition of multiple layers of oppositely charged polyelectrolytes on spherical, colloidal templates. These templates can be decomposed by coating multiple layers, resulting in hollow shells. In this paper, we describe a novel drug delivery system for loading photosensitizer drugs into hollow multilayered microcapsules for photoprocess applications. Methods: Manganese carbonate particles were prepared by mixing NH4HCO3 and MnSO4 and performing consecutive polyelectrolyte adsorption processes onto these templates using poly-(sodium 4-styrene sulfonate) and poly-(allylamine hydrocholoride). A photosensitizer was also incorporated into the layers. Hollow spheres were fabricated by removing the cores in the acidic solution. The hollow, multilayered microcapsules were studied by scanning electron microscopy, steady-state, and time-resolved techniques. Their biological activity was evaluated in vitro with cancer cells using a conventional MTT assay. Results: The synthesized CaCO3 microparticles were uniform, non-aggregated, and highly porous spheres. The phthalocyanine derivatives loaded in the microcapsules maintained their photophysical behaviour after encapsulation. The spectroscopic results presented here showed excellent photophysical behaviour of the studied drug. We observed a desirable increase in singlet oxygen production, which is favourable for the PDT protocol. Cell viability after treatment was determined and the proposed microcapsules caused 80% cell death compared to the control. Conclusions: The results demonstrate that photosensitizer adsorption into the CaCO3 microparticle voids together with the layer-by-layer assembly of biopolymers provide a method for the fabrication of biocompatible microcapsules for use as biomaterials. (AU)

Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/51729-5 - Estudos de fármacos fotossensíveis em modelos tridimensionais de pele em matriz de colágeno: avaliação da ação fotodinâmica no processo de cicatrização tecidual
Beneficiário:Andreza Ribeiro Simioni
Linha de fomento: Bolsas no Brasil - Pós-Doutorado